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Goat Anti-Human Glial-Derived Neurotrophic Factor (GDNF)
Antigen Affinity Purified
(ATF-1, ATF2, HFB1-GDNF)
Polyclonal Antibody
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Prod. No.:G116
Host:Goat
Pkg. Size:100 µg, 1 mg
Storage:-20°C to -70°C Detailed storage instructions below.
Description
Specificity:
Rabbit Anti-Human Glial-Derived Neurotrophic Factor (GDNF) recognizes Human GDNF. This antigen affinity purified polyclonal antibody was purified using a proprietary chromatographic technique that includes covalently immobilizing the antigen proteins or peptides to agarose based beads. This purification method enhances specificity, reduces nonspecific binding of extraneous IgG and provides you with the most reliable reagent available for your early discovery research.

Background:
Glial cell derived neurotrophic factor, also known as GDNF is a glycosylated, disulfide-bonded homodimer that is a distantly related member of the transforming growth factor-beta superfamily.1 GDNF potently promotes the survival of many types of neurons, most prominently, dopaminergic and motorneurons. GDNF regulates kidney development and spermatogenesis, and it affects alcohol consumption.2

Known Species Cross Reactivity: Hu+
Immunogen
Purified Recombinant Human GDNF (>98%)
Formulation
This antigen affinity purified polyclonal antibody has been 0.2 µm filtered and lyophilized from modified Dulbecco’s phosphate buffered saline (1X PBS) pH 7.2 – 7.3 containing 5.0% w/v trehalose with no calcium, magnesium, or preservatives present.
Reconstitution
We suggest aseptically reconstituting this lyophilized, antigen affinity purified polyclonal antibody with 1 ml of sterile 1X PBS pH 7.2-7.3. This will result in a final antibody concentration of 0.1 mg/ml.
Endotoxin
<0.1 EU/µg as determined by the LAL method
Storage and Stability
The lyophilized antigen affinity purified polyclonal antibody can be stored desiccated at -20°C to -70°C for twelve months from date of receipt. The reconstituted antibody can be stored for at least four weeks at 2-8°C. For long-term storage of the reconstituted antibody, aseptically aliquot into working volumes and store at -20°C to -70°C in a manual defrost freezer. Avoid Repeated Freeze Thaw Cycles. No detectable loss of activity was observed after six months.
Reported Applications
Indirect ELISA, IHC (Frozen), N, WB
Each investigator should determine their own optimal working dilution for specific applications.


Country of Origin
USA
References
1. Collins F. et al. (1993)Science 260: 1130
2. Ron D et al. (2008) Proc. Natl. Acad. Sci. U.S.A. 105: 8114
Related Products
Recombinant Human GDNF (Leinco Prod. No.: G120)
Recombinant Rat GDNF (Leinco Prod. No.: G639)
Anti-Human GDNF-Biotin (Antigen Aff. Pur.) (Leinco Prod. No.: G141)
Anti-Human GDNF (Leinco Prod. No.: G663)
Recombinant Murine GDNF (Leinco Prod. No.: G638)
Recombinant Human GDNF (produced from NSO Cells) (Leinco Prod. No.: G715)
Products are for research use only. Not for use in diagnostic or therapeutic procedures.
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