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Goat Anti-Mouse Granulocyte Colony Stimulating Factor (G-CSF)
Antigen Affinity Purified
(CSF-3, MGI-1G, GM-CSF Beta, Pluripoietin)
Polyclonal Antibody
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Prod. No.:G134
Host:Goat
Pkg. Size:100 µg, 1.0 mg
Storage:-20°C to -70°C Detailed storage instructions below.
Description
Specificity:
Rabbit Anti-Mouse Granulocyte Colony Stimulating Factor (G-CSF) recognizes Mouse G-CSF. This antigen affinity purified polyclonal antibody was purified using a proprietary chromatographic technique that includes covalently immobilizing the antigen proteins or peptides to agarose based beads. This purification method enhances specificity, reduces nonspecific binding of extraneous IgG and provides you with the most reliable reagent available for your early discovery research.

Background:
Granulocyte colony-stimulating factor (G-CSF or GCSF), also known as G-CSF colony-stimulating factor 3 (CSF 3) is a colony-stimulating factor hormone. It is a glycoprotein, growth factor or cytokine produced by a number of different tissues to stimulate the bone marrow to produce granulocytes and stem cells. G-CSF then stimulates the bone marrow to release them into the blood. It also stimulates the survival, proliferation, differentiation, and function of neutrophil precursors and mature neutrophils. G-CSF is produced by endothelium, macrophages, and a number of other immune cells. It stimulates the production of white blood cells. G-CSF is also used to increase the number of hematopoietic stem cells in the blood of the donor before collection by leukapheresis for use in hematopoietic stem cell transplantation.

Known Species Cross Reactivity: Ms+
Immunogen
Purified Recombinant Mouse G-CSF (>98%)
Formulation
This antigen affinity purified polyclonal antibody has been 0.2 µm filtered and lyophilized from modified Dulbecco’s phosphate buffered saline (1X PBS) pH 7.2 – 7.3 containing 5.0% w/v trehalose with no calcium, magnesium, or preservatives present.
Reconstitution
We suggest aseptically reconstituting this lyophilized, antigen affinity purified polyclonal antibody with 1 ml of sterile 1X PBS pH 7.2-7.3. This will result in a final antibody concentration of 0.1 mg/ml.
Endotoxin
<0.1 EU/µg as determined by the LAL method
Storage and Stability
The lyophilized antigen affinity purified polyclonal antibody can be stored desiccated at -20°C to -70°C for twelve months from date of receipt. The reconstituted antibody can be stored for at least four weeks at 2-8°C. For long-term storage of the reconstituted antibody, aseptically aliquot into working volumes and store at -20°C to -70°C in a manual defrost freezer. Avoid Repeated Freeze Thaw Cycles. No detectable loss of activity was observed after six months.
Reported Applications
Indirect ELISA, N, WB
Each investigator should determine their own optimal working dilution for specific applications.


Country of Origin
USA
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Recombinant Mouse G-CSF (Leinco Prod. No.: G129)
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Products are for research use only. Not for use in diagnostic or therapeutic procedures.
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