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Goat Anti-Human B7 Homolog 2 (B7-H2)
Antigen Affinity Purified
(B7RP-1, B7h, LICOS, GL50)
Polyclonal Antibody
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Prod. No.:B563
Host:Goat
Pkg. Size:100 µg, 1 mg
Storage:-20°C to -70°C Detailed storage instructions below.
Description
Specificity:
Goat Anti-Human B7 Homolog 2 (B7-H2) recognizes Human B7-H2. This antigen affinity purified polyclonal antibody was purified using a proprietary chromatographic technique that includes covalently immobilizing the antigen proteins or peptides to agarose based beads. This purification method enhances specificity, reduces nonspecific binding of extraneous IgG and provides you with the most reliable reagent available for your early discovery research.

Background:
Human B7-H2, also called B7RP-1, B7h, LICOS, and GL50, is a member of the growing B7 family of immune costimulatory proteins. Other family members include B7-1, B7-2, B7-H1 (PD-L1), PD-L2, and B7-H3. B7 proteins are members of the immunoglobulin (Ig) superfamily, the extracellular domains contain 2 Ig-like domains and all members have short cytoplasmic domains. Among the family members, they share about 20 - 25% amino acid identity. Human and mouse B7-H2 share approximately 49% amino acid identity. B7-H2 has been identified as the ligand for ICOS, a member of the CD28 family of costimulatory receptors. The B7-H2/ICOS interaction appears to play roles in T cell dependent B cell activation and Th differentiation.

Known Species Cross Reactivity: Hu+
Immunogen
Purified Recombinant Human B7-H2 (>98%)
Formulation
This antigen affinity purified polyclonal antibody has been 0.2 µm filtered and lyophilized from modified Dulbecco’s phosphate buffered saline (1X PBS) pH 7.2 – 7.3 containing 5.0% w/v trehalose with no calcium, magnesium, or preservatives present.
Reconstitution
We suggest aseptically reconstituting this lyophilized, antigen affinity purified polyclonal antibody with 1.0 µl of sterile 1X PBS pH 7.2-7.3. This will result in a final antibody concentration of 0.1 mg/ml.
Endotoxin
<0.1 EU/µg as determined by the LAL method
Storage and Stability
The lyophilized antigen affinity purified polyclonal antibody can be stored desiccated at -20°C to -70°C for twelve months from date of receipt. The reconstituted antibody can be stored for at least four weeks at 2-8°C. For long-term storage of the reconstituted antibody, aseptically aliquot into working volumes and store at -20°C to -70°C in a manual defrost freezer. Avoid Repeated Freeze Thaw Cycles. No detectable loss of activity was observed after six months.
Reported Applications
Indirect ELISA, FC, WB
Indirect ELISA: This antibody can be used in an indirect detection ELISA at 0.5-1.0 µg/ml with a suitable second step reagent such as Donkey Anti-Goat IgG (H&L)-Horseradish Peroxidase (Leinco Prod. No.: G505). The detection sensitivity of this indirect ELISA for Human B7-H2 is approximately 0.3 ng/well.
Flow Cytometry: It is recommended to use the indirect method for signal enhancement when enumerating cells expressing B7-H2. A suggested method would be to stain cells expressing B7-H2 with 10 µg per 1 - 2.5 x 105 cells in a total staining volume of 200 µl followed by Donkey F(ab')2 Anti-Goat IgG (H&L)-R-phycoerythrin (R-PE) (Leinco Prod. No.: G641).
Western Blotting: To detect Human B7-H2 this polyclonal antibody can be used at a concentration of 0.1-0.2 µg/ml. This polyclonal antibody should be used in conjunction with compatible second-step reagents such as Donkey Anti-Goat IgG (H&L)-Horseradish Peroxidase (Leinco Prod. No.: G505) and a chromogenic substrate such as TMB HRPO Membrane/IHC Substrate UltraSensitive™ One Component "Ready Use" (Leinco Prod. No.: T343). The detection limit for Human B7-H2 is 0.5 ng/lane under either reducing or non-reducing conditions. The sensitivity of detection may increase up to 50 fold when a chemiluminescent substrate is used. A suitable Western blotting control is Recombinant Human B7-H2 (Leinco Prod. No.: B537).
Each investigator should determine their own optimal working dilution for specific applications.


Country of Origin
USA
Related Products
Donkey Anti-Goat IgG (H&L)-Horseradish Peroxidase (Leinco Prod. No.: G505)
Donkey F(ab')2 Anti-Goat IgG (H&L)-R-phycoerythrin (R-PE) (Leinco Prod. No.: G641)
TMB HRPO Membrane/IHC Substrate UltraSensitive™ One Component "Ready Use" (Leinco Prod. No.: T343)
Recombinant Human B7-H2 (Leinco Prod. No.: B537)
Recombinant Human B7-H2/Fc Chimera, CF (Leinco Prod. No.: B528)
Anti-Mouse B7-H2 (Antigen Aff. Pur.) (Leinco Prod. No.: B562)
Anti-Human B7-H2 (Leinco Prod. No.: B569)
Anti-Mouse B7-H2-Biotin (Antigen Aff. Pur.) (Leinco Prod. No.: B606)
Anti-Human B7-H2-Biotin (Antigen Aff. Pur.) (Leinco Prod. No.: B607)
Recombinant Mouse B7-H2 (Leinco Prod. No.: B536)
Products are for research use only. Not for use in diagnostic or therapeutic procedures.
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