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Goat Anti-Rat Inter-Cellular Adhesion Molecule 1 (ICAM-1)
Antigen Affinity Purified
(BB2, CD54, P3.58)
Polyclonal Antibody
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Prod. No.:I-727
Host:Goat
Pkg. Size:100 µg, 1 mg
Storage:-20°C to -70°C Detailed storage instructions below.
Description
Specificity:
Goat Anti-Rat Intercellular Adhesion Molecule 1 (ICAM-1) recognizes Rat ICAM-1. This antigen affinity purified polyclonal antibody was purified using a proprietary chromatographic technique that includes covalently immobilizing the antigen proteins or peptides to agarose based beads. This purification method enhances specificity, reduces nonspecific binding of extraneous IgG and provides you with the most reliable reagent available for your early discovery research.

Background:
Intercellular adhesion molecule-1 (ICAM-1) is one of an important cell adhesion molecules (CAMs) family transmembrane glycoprotein1 and is critical for the firm arrest and transmigration of leukocytes out of blood vessels and into tissues. ICAM-1 is constitutively present on endothelial cells, but its expression is increased by proinflammatory cytokines.2 ICAM-1 is a marker of endothelial dysfunction leading to damaging vascular disorders, in umbilical and placental vascular tissue of gestational pregnancies.3

Immunogen
NS0-Derived Recombinant Rat ICAM-1 Extracellular Domain
Formulation
This antigen affinity purified polyclonal antibody has been 0.2 µm filtered and lyophilized from modified Dulbecco’s phosphate buffered saline (1X PBS) pH 7.2 – 7.3 containing 5.0% w/v trehalose with no calcium, magnesium, or preservatives present.
Reconstitution
We suggest aseptically reconstituting this lyophilized, antigen affinity purified polyclonal antibody with 1 µl of sterile 1X PBS pH 7.2-7.3. This will result in a final antibody concentration of 0.1 mg/ml.
Endotoxin
<0.1 EU/µg as determined by the LAL method
Storage and Stability
The lyophilized antigen affinity purified polyclonal antibody can be stored desiccated at -20°C to -70°C for twelve months from date of receipt. The reconstituted antibody can be stored for at least four weeks at 2-8°C. For long-term storage of the reconstituted antibody, aseptically aliquot into working volumes and store at -20°C to -70°C in a manual defrost freezer. Avoid Repeated Freeze Thaw Cycles. No detectable loss of activity was observed after six months.
Reported Applications
Indirect ELISA, IHC (NBF/Par.), WB
Indirect ELISA: This antibody can be used in an indirect detection ELISA at 0.5 - 1.0 µg/ml with a suitable second step reagent such as Donkey Anti-Goat IgG (H&L)-Horseradish Peroxidase (Leinco Prod. No.: G505). The detection sensitivity of this indirect ELISA for Rat ICAM-1 is approximately 0.1 ng/well.
IHC (NBF/Par.): This antibody should give satisfactory staining results when used at a concentration of 2-5 µg/ml. The recommended secondary antibody for IHC is Donkey Anti-Goat IgG (H&L)-Horseradish Peroxidase (Leinco Prod. No.: G505). For chromogenic detection with high signal and low background use 3, 3'-Diaminobenzidine Tetrahydrochloride (DAB) Substrate Kit (Leinco Prod. No.: D100) or Optional Metal Enhanced DAB Substrate Kit (Leinco Prod. No.: K107).
Western Blotting: To detect Rat ICAM-1 this polyclonal antibody can be used at a concentration of 0.1 - 0.2 µg/ml. This polyclonal antibody should be used in conjunction with compatible second-step reagents such as Donkey Anti-Goat IgG (H&L)-Horseradish Peroxidase (Leinco Prod. No.: G505) and a chromogenic substrate such as TMB HRPO Membrane/IHC Substrate UltraSensitive™ One Component "Ready Use" (Leinco Prod. No.: T343). The detection limit for Rat ICAM-1 is 5 ng/lane under either reducing or non-reducing conditions. The sensitivity of detection may increase up to 50 fold when a chemiluminescent substrate is used.
Each investigator should determine their own optimal working dilution for specific applications.


Country of Origin
USA
References
1. Li S et al. (2009) Biochem Biophys Res Commun. 381: 459
2. Wolf S et al. (2009) Pharmacol Rep 61: 22
3. Ozcan U et al. (2009) Arch Gynecol Obstet.
Related Products
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3, 3'-Diaminobenzidine Tetrahydrochloride (DAB) Substrate Kit (Leinco Prod. No.: D100)
Optional Metal Enhanced DAB Substrate Kit (Leinco Prod. No.: K107)
TMB HRPO Membrane/IHC Substrate UltraSensitive™ One Component "Ready Use" (Leinco Prod. No.: T343)
Anti-Human ICAM-1 Purified (Leinco Prod. No.: C167)
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Recombinant Human ICAM-1 (Leinco Prod. No.: I-580)
Recombinant Mouse ICAM-1 (Leinco Prod. No.: I-587)
Anti-Mouse ICAM-1-Allophycocyanin (Leinco Prod. No.: C354)
Anti-Mouse ICAM-1-Fluorescein (Leinco Prod. No.: C269)
Anti-Mouse ICAM-1-Biotin (Leinco Prod. No.: C268)
Anti-Mouse ICAM-1-R-phycoerythrin (Leinco Prod. No.: C270)
Anti-Human ICAM-1 Purified Low Endotoxin Functional Formulation (Leinco Prod. No.: C372)
Anti-Mouse ICAM-1 Low Endotoxin Functional Formulation (Leinco Prod. No.: C391)
Anti-Human ICAM-1-Allophycocyanin (APC) (Leinco Prod. No.: C323)
Anti-Human ICAM-1-Biotin (Leinco Prod. No.: C168)
PN:
Anti-Human ICAM-1-R-phycoerythrin (R-PE) (Leinco Prod. No.: C170)
Anti-Mouse ICAM-1 Purified (Leinco Prod. No.: C267)
Products are for research use only. Not for use in diagnostic or therapeutic procedures.
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