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Goat Anti-Human Interferon Gamma (IFN-γ)
Antigen Affinity Purified
(Immune Interferon, Type II Interferon, T Cell Interferon, MAF, IFNG, IFG, IFI)
Polyclonal Antibody
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Prod. No.:I-141
Host:Goat
Pkg. Size:100 µg, 1 mg
Storage:-20°C to -70°C Detailed storage instructions below.
Description
Specificity:
Rabbit Anti-Human Interferon-Gamma (IFN-γ) recognizes Human IFN-γ. This antigen affinity purified polyclonal antibody was purified using a proprietary chromatographic technique that includes covalently immobilizing the antigen proteins or peptides to agarose based beads. This purification method enhances specificity, reduces nonspecific binding of extraneous IgG and provides you with the most reliable reagent available for your early discovery research.

Background:
Interferon-gamma (IFN-γ) or type II interferon is a dimerized soluble cytokine that is the only member of the type II class of interferons.1 It is a cytokine critical for innate and adaptive immunity against viral and intracellular bacterial infections and for tumor control. IFNG is produced predominantly by natural killer (NK) and natural killer T (NKT) cells as part of the innate immune response, and by CD4 and CD8 cytotoxic T lymphocyte (CTL) effector T cells once antigen-specific immunity develops.2 IFN-γ has antiviral, immunoregulatory, and anti-tumour properties.3

Known Species Cross Reactivity: Hu+
Immunogen
Purified Recombinant Human IFN-γ (>98%)
Formulation
This antigen affinity purified polyclonal antibody has been 0.2 µm filtered and lyophilized from modified Dulbecco’s phosphate buffered saline (1X PBS) pH 7.2 – 7.3 containing 5.0% w/v trehalose with no calcium, magnesium, or preservatives present.
Reconstitution
We suggest aseptically reconstituting this lyophilized, antigen affinity purified polyclonal antibody with 1 ml of sterile 1X PBS pH 7.2-7.3. This will result in a final antibody concentration of 0.1 mg/ml.
Endotoxin
<0.1 EU/µg as determined by the LAL method
Storage and Stability
The lyophilized antigen affinity purified polyclonal antibody can be stored desiccated at -20°C to -70°C for twelve months from date of receipt. The reconstituted antibody can be stored for at least four weeks at 2-8°C. For long-term storage of the reconstituted antibody, aseptically aliquot into working volumes and store at -20°C to -70°C in a manual defrost freezer. Avoid Repeated Freeze Thaw Cycles. No detectable loss of activity was observed after six months.
Reported Applications
Indirect ELISA, IHC (NBF/Par.), N, WB
Indirect ELISA: This antibody can be used in an indirect detection ELISA at 0.5-1.0 µg/ml with a suitable second step reagent such as Donkey Anti-Goat IgG (H&L)-Horseradish Peroxidase (Leinco Prod. No.: G505). The detection sensitivity of this indirect ELISA for Human IFN-γ is approximately 7 ng/well.
IHC (NBF/Par.): This antibody should give satisfactory staining results when used at a concentration of 5-15 µg/ml. The recommended secondary antibody for IHC is Donkey Anti-Goat IgG (H&L)-Horseradish Peroxidase (Leinco Prod. No.: G505). For chromogenic detection with high signal and low background use 3, 3'-Diaminobenzidine Tetrahydrochloride (DAB) Substrate Kit (Leinco Prod. No.: D100) or Optional Metal Enhanced DAB Substrate Kit (Leinco Prod. No.: K107).
Neutralization: This antibody is useful for neutralization of Human IFN-γ bioactivity. The antibody dose required to neutralize 50% (ND50) of the biological activity of Human IFN-γ (at 5 ng/ml) is 0.1-0.3 µg/ml.
Western Blotting: To detect Human IFN-γ this polyclonal antibody can be used at a concentration of 0.1-0.2 µg/ml. This polyclonal antibody should be used in conjunction with compatible second-step reagents such as Donkey Anti-Goat IgG (H&L)-Horseradish Peroxidase (Leinco Prod. No.: G505) and a chromogenic substrate such as TMB HRPO Membrane/IHC Substrate UltraSensitive™ One Component "Ready Use" (Leinco Prod. No.: T343). The detection limit for Human IFN-γ is 2.0 ng/lane under either reducing or non-reducing conditions. The sensitivity of detection may increase up to 50 fold when a chemiluminescent substrate is used. A suitable Western blotting control is Recombinant Human IFN-γ (Leinco Prod. No.: I-179).
Each investigator should determine their own optimal working dilution for specific applications.


Country of Origin
USA
References
1. Goeddel DV et al. (1982) Nature 298: 859
2. Wilson CB et al. (2007) Adv. Immunol. 96: 41
3. Hume DA et al. (2004) J Leukoc Biol. 75: 163
Related Products
Donkey Anti-Goat IgG (H&L)-Horseradish Peroxidase (Leinco Prod. No.: G505)
3, 3'-Diaminobenzidine Tetrahydrochloride (DAB) Substrate Kit (Leinco Prod. No.: D100)
Optional Metal Enhanced DAB Substrate Kit (Leinco Prod. No.: K107)
TMB HRPO Membrane/IHC Substrate UltraSensitive™ One Component "Ready Use" (Leinco Prod. No.: T343)
Recombinant Human IFN-γ (Leinco Prod. No.: I-179)
Anti-Mouse IFN-γ-Biotin (Antigen Aff. Pur.) (Leinco Prod. No.: I-201)
Recombinant Mouse IFN-γ (Leinco Prod. No.: I-217)
Anti-Rat IFN-γ (Antigen Aff. Pur.) (Leinco Prod. No.: I-233)
Anti-Human IFN-γ (Leinco Prod. No.: I-328)
Anti-Human IFN-γ (Leinco Prod. No.: I-646)
Anti-Human IFN-γ (Leinco Prod. No.: I-647)
Anti-Mouse IFN (Leinco Prod. No.: I-669)
Anti-Rat IFN-γ (Leinco Prod. No.: I-683)
Mouse IFN-γ ELISA Development Kit (Leinco Prod. No.: I-297)
Rat IFN-γ ELISA Development Kit (Leinco Prod. No.: I-381)
Anti-Mouse IFN-γ (Antigen Aff. Pur.) (Leinco Prod. No.: I-232)
Anti-Human IFN-γ-Biotin (Antigen Aff. Pur.) (Leinco Prod. No.: I-178)
Anti-Rat IFN-γ-Biotin (Antigen Aff. Pur.) (Leinco Prod. No.: I-203)
Recombinant Rat IFN-γ (Leinco Prod. No.: I-223)
Human IFN-γ ELISA Development Kit (Leinco Prod. No.: I-296)
Anti-Mouse IFN-γ Low Endotoxin Functional Formulation (Leinco Prod. No.: I-438)
PN:
Products are for research use only. Not for use in diagnostic or therapeutic procedures.
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