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Rabbit Anti-Human NALP2
Purified No Carrier Protein
(NACHT Domain-, Leucine-Rich-Repeat-, and PYD-Containing Protein 2, PYRIN-Containing APAF1-Like Protein2, PAN1, PYPAF2 )
Polyclonal Antibody
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Prod. No.:N158
Host:Rabbit
Conc.:0.5 mg/ml
Pkg. Size:0.1 mg, 0.5 mg, 1 mg
Storage:2-8°C Detailed storage instructions below.
Description
Specificity:
Rabbit Anti-Human NALP2 recognizes Human, Mouse and Rat NALP2. This polyclonal antibody was purified using affinity chromatography.

Background:
NALP2 belongs to a family of cytoplasmic proteins that have been implicated in cell responses to apoptotic and inflammatory stimuli.1 Unlike the prototypical NALP protein NALP1, NALP2 only contains a NACHT domain, leucine rich repeat (LRR), and pyrin-containing domain (PYD).2 This protein interacts with the adapter protein ASC in addition to CARD8 and caspase-1 to form an inflammasome with high proIL-1Β-processing activity and is thought to function as a modulator of NF-κB and procaspase-1 activation in macrophages.2,3 It has also been suggested that NALP2, in addition to other NALP family members, can act as innate sensors of pathogens in a manner similar to the toll-like receptors (TLRs).4 At least two alternatively spliced transcript variants encoding distinct isoforms have been found for NALP2.

Known Species Cross Reactivity: Ms+, Hu+, Rt+
Immunogen
NALP2 Blocking Peptide (Leinco Prod. No.: N171)
Blocking Peptide
NALP2 Blocking Peptide (Leinco Prod. No.: N171)
Formulation
This polyclonal antibody is formulated in phosphate buffered saline (PBS) pH 7.4 containing 0.02% sodium azide as a preservative.
Storage and Stability
This polyclonal antibody is stable for at least one week when stored at 2-8°C. For long term storage, aliquot in working volumes without diluting and store at –20°C in a manual defrost freezer. Avoid Repeated Freeze Thaw Cycles.
Reported Applications
WB
Western Blotting: This antibody can be used to detect Human, Mouse and Rat NALP2 by Western blot analysis at a concentration of 1.0-2.0 µg/ml when used in conjunction with compatible secondary reagents, such as Goat Anti-Rabbit IgG (H&L)-Horseradish Peroxidase (Leinco Prod. No.: R1190), under either reducing or non-reducing conditions. The positive control for Western blotting is PC-3 Whole Cell Lysate (Leinco Prod. No.: P166).
Each investigator should determine their own optimal working dilution for specific applications.


Country of Origin
USA
References
1. Tschopp, J. et al. (2003) Nat. Rev. Mol. Cell Biol. 4:95
2. Bruey, J. M. et al. (2004) J. Biol. Chem. 279:51897
3. Agostini, L. et al. (2004) Immunity 20:319
4. Martinon, F. et al. (2005) TRENDS Imm. 26:447
Related Products
NALP2 Blocking Peptide (Leinco Prod. No.: N171)
Goat Anti-Rabbit IgG (H&L)-Horseradish Peroxidase (Leinco Prod. No.: R1190)
PC-3 Whole Cell Lysate (Leinco Prod. No.: P166)
Products are for research use only. Not for use in diagnostic or therapeutic procedures.
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