Anti-Human NIK (CT) [N131] : Leinco Technologies, excellence in early discovery research

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Rabbit Anti-Human NIK

Purified No Carrier Protein

(NF-κB Inducing Kinase )

Polyclonal Antibody

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Prod. No.:	N131
Host:	Rabbit
Conc.:	0.5 mg/ml
Pkg. Size:	0.1 mg, 0.5 mg, 1 mg
Storage:	2-8°C Detailed storage instructions below.

Description

Specificity:

Rabbit Anti-Human NIK recognizes an epitope near the C-terminus of Human NIK. This polyclonal antibody was purified using affinity chromatography.

Background:

Nuclear factor κ B (NF-κB) is a ubiquitous transcription factor and an essential mediator of gene expression during activation of immune and inflammatory responses. NF-κB mediates the expression of a great variety of genes in response to extracellular stimuli including IL-1, TNFα, LPS and mitogens. A serine/threonine protein kinase which mediates NF-κB activation by IL-1, TNFα and CD95 was identified recently and designated NIK (for NF-κB inducing kinase)(1). NIK is an activator of IκB kinase α and β (IKKα and IKKβ)(2-5). Therefore, NIK is a key molecule in the NF-κB signaling pathway leading to the induction of a variety of gene expression in response to proinflammatory cytokines and bacteria products.

Known Species Cross Reactivity: Hu+

Immunogen

NIK (CT) Blocking Peptide (Leinco Prod. No.: N134)

Blocking Peptide

NIK (CT) Blocking Peptide (Leinco Prod. No.: N134)

Formulation

This polyclonal antibody is formulated in phosphate buffered saline (PBS) pH 7.4 containing 0.02% sodium azide as a preservative.

Storage and Stability

This polyclonal antibody is stable for at least one week when stored at 2-8°C. For long term storage, aliquot in working volumes without diluting and store at –20°C in a manual defrost freezer. Avoid Repeated Freeze Thaw Cycles.

Reported Applications

Western Blotting: This antibody can be used to detect Human NIK by Western blot analysis at a concentration of µg/ml when used in conjunction with compatible secondary reagents, such as Goat Anti-Rabbit IgG (H&L)-Horseradish Peroxidase (Leinco Prod. No.: R1190), under either reducing or non-reducing conditions. The positive control for Western blotting is 293 Whole Cell Lysate (Leinco Prod. No.: A182).

Each investigator should determine their own optimal working dilution for specific applications.

Country of Origin

USA

References

1. Malinin NL, Boldin MP, Kovalenko AV, Wallach . MAP3Krelated kinase involved in NF-kappaB induction by TNF, CD95 and IL-1. Nature 1997;385:540-544

2. Regnier CH, Song HY, Gao X, Goeddel DV, Cao Z, Rothe M. Identification and characterization of an IkappaB kinase. Cell 1997;90:373-383

3. Woronicz JD, Gao X, Cao Z, Rothe M, Goeddel DV. IkappaB kinase-beta: NF-kappaB activation and complex formation with IkappaB kinase-alpha and NIK. Science 1997;278:866-869

4. Ling L, Cao Z, Goeddel D. NF-kappaB-inducing kinase activates IKK-alpha by phosphorylation of Ser-176. Proc Natl Acad Sci USA 1998;95:3792-3797

5. Nakano H, Shindo M, Sakon S, Nishinaka S, Mihara M, Yagita H, Okumura K. Differential regulation of IkappaB kinase alpha and beta by two upstream kinases, NF-kappaB-inducing kinase and mitogen-activated protein kinase/ERK kinase kinase-1. Proc Natl Acad Sci USA 1998;95:3537-3542 (RD0400)