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Rabbit Anti-Human TNF Receptor-Associated Factor 3 (TRAF3)
Purified No Carrier Protein
(CD40 Binding Protein, LMP1 Associated Protein 1, CD40BP, LAP)
Polyclonal Antibody
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Prod. No.:T284
Host:Rabbit
Conc.:0.5 mg/ml
Pkg. Size:0.1 mg, 0.5 mg, 1 mg
Storage:2-8°C Detailed storage instructions below.
Description
Specificity:
Rabbit Anti-Human TNF Receptor-Associated Factor 3 (TRAF3) recognizes an epitope near the C-terminus of Human and Mouse TRAF3. This polyclonal antibody was purified using affinity chromatography.

Background:
Tumor necrosis factor (TNF) receptor associated factors (TRAFs) are the major signal transducers for the TNF receptor superfamily and the interleukin-1 receptor/Toll-like receptor (IL-1/TLR) superfamily.1 TRAF3 was first identified by its interaction with CD40 and the Epstein-Barr virus transforming protein LMP1.2,3 Several TRAF3 mRNA splice variants exist and some of these can activate the transcription factor NF-κB.4 Besides CD40, TRAF3 also interacts with the TRFR superfamily member lymphotoxin-β receptor (LTβR) in association with TRAF2 and the apoptosis inhibitors cIAP1 and Smac. It has been suggested that TRAF3 induces mitochondria-mediated apoptosis upon binding of the TNF family cytokine LIGHT by LTβR.

Known Species Cross Reactivity: Ms+, Hu+
Formulation
This polyclonal antibody is formulated in phosphate buffered saline (PBS) pH 7.4 containing 0.02% sodium azide as a preservative.
Storage and Stability
This polyclonal antibody is stable for at least one week when stored at 2-8°C. For long term storage, aliquot in working volumes without diluting and store at –20°C in a manual defrost freezer. Avoid Repeated Freeze Thaw Cycles.
Reported Applications
WB
Western Blotting: This antibody can be used to detect Human and Mouse TRAF3 by Western blot analysis at a concentration of 1.0-2.0 µg/ml when used in conjunction with compatible secondary reagents, such as Goat Anti-Rabbit IgG (H&L)-Horseradish Peroxidase (Leinco Prod. No.: R1190), under either reducing or non-reducing conditions. The positive control for Western blotting is 3T3 Balb Whole Cell Lysate (Leinco Prod. No.: A183).
Each investigator should determine their own optimal working dilution for specific applications.


Country of Origin
USA
References
1. Arch, R. H. et al. (1998) Genes Dev. 12:2821
2. Cheng, G. et al. (1995) Science 267:1494
3. Mosialos, G. et al. (1995) Cell 80:389
4. van Eyndhoven, W. G. et al. (1999) Mol. Immunol. 36:647
Related Products
Goat Anti-Rabbit IgG (H&L)-Horseradish Peroxidase (Leinco Prod. No.: R1190)
3T3 Balb Whole Cell Lysate (Leinco Prod. No.: A183)
Anti-Human TNF Receptor-Associated Factor 3 (CT-2) (TRAF3) (Leinco Prod. No.: T285)
TNF Receptor-Associated Factor 3 (CT) Blocking Peptide (Leinco Prod. No.: T304)
TNF Receptor-Associated Factor 3 (NT) Blocking Peptide (Leinco Prod. No.: T314)
Products are for research use only. Not for use in diagnostic or therapeutic procedures.
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