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Rabbit Anti-Human TRIM5α
Purified No Carrier Protein
Polyclonal Antibody
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Prod. No.:T333
Host:Rabbit
Conc.:0.5 mg/ml
Pkg. Size:0.1 mg, 0.5 mg, 1 mg
Storage:2-8°C Detailed storage instructions below.
Description
Specificity:
Rabbit Anti-Human TRIM5α recognizes an epitope in the intermediate domain of Human TRIM5α. This polyclonal antibody was purified using affinity chromatography.

Background:
TRIM5 is a member of a broad family of otherwise unrelated proteins defined by the presence of a tripartite motif containing a RING domain, a B-box type 1, and a B-box type 2, followed by a coiled-coil region.1 TRIM5 has six alternately spliced isoforms, the longest of which is the variant which also contains a carboxy-terminal B30.2 (SPRY) domain.1 Expression of TRIM5α variants from humans, rhesus monkeys, and African green monkeys enabled resistance to infection by various retroviruses including HIV-1,2,3 albeit at differing efficiencies. All TRIM5α variants could inhibit at least two different retroviruses, but not from those viruses isolated from the same species, suggesting that TRIM5α acts as a natural barrier to cross-species retrovirus transmission.3

Known Species Cross Reactivity: Hu+
Formulation
This polyclonal antibody is formulated in phosphate buffered saline (PBS) pH 7.4 containing 0.02% sodium azide as a preservative.
Storage and Stability
This polyclonal antibody is stable for at least one week when stored at 2-8°C. For long term storage, aliquot in working volumes without diluting and store at –20°C in a manual defrost freezer. Avoid Repeated Freeze Thaw Cycles.
Reported Applications
IHC (NBF/Par.), WB
Each investigator should determine their own optimal working dilution for specific applications.


Country of Origin
USA
References
1. Reymond, A. et al. (2001) EMBO J. 20:2140
2. Stremlau, M. et al. (2004) Nature 427:848
3. Hatziioannou, T. et al. (2004) Proc. Nat’l. Acad. Sci. USA 101:10774
Related Products
Anti-Human TRIM5α (CT) (Leinco Prod. No.: T331)
Anti-Human TRIM5α (Intermediate Domain 1) (Leinco Prod. No.: T332)
TRIM5a (CT) Blocking Peptide (Leinco Prod. No.: T336)
Trim5a (Intermediate Domain - 1) Blocking Peptide (Leinco Prod. No.: T337)
TRIM5a (Intermediate Domain - 2) Blocking Peptide (Leinco Prod. No.: T338)
Products are for research use only. Not for use in diagnostic or therapeutic procedures.
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