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Goat Anti-Human gAcrp30/Adipolean

Antigen Affinity Purified

(apm-1, Adipolean (human) )

Polyclonal Antibody

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Prod. No.:	A223
Host:	Goat
Pkg. Size:	100 µg, 1 mg
Storage:	-20°C Detailed storage instructions below.

Description

Specificity:

Goat Anti-Human gAcrp30/Adipolean recognizes Human gAcrp30/Adipolean. This antigen affinity purified polyclonal antibody was purified using a proprietary chromatographic technique that includes covalently immobilizing the antigen proteins or peptides to agarose based beads. This purification method enhances specificity, reduces nonspecific binding of extraneous IgG and provides you with the most reliable reagent available for your early discovery research.

Background:

Human gAcrp30 is a naturally occurring globular protein, obtained by proteolytic processing of adiponectin. Adiponectin is produced and secreted exclusively by adipocytes, and is a relatively abundant plasma protein, accounting for up to 0.05% of total serum protein. Like Adiponectin, gAcrp30 is capable of decreasing hyperglycemia and reversing insulin resistance. Additionally, gAcrp30 has been shown to be an important factor in promoting fat loss by signaling muscle to absorb and burn Free-Fatty Acids (FFAs). The signaling receptors for adiponectin and gAcrp30 have recently been identified and names AdipoR1 and AdipoR2. AdipoR2 is predominantly expressed in the liver.

Known Species Cross Reactivity: Hu+

Immunogen

Purified Recombinant Human gAcrp30/Adipolean (>98%)

Formulation

This antigen affinity purified polyclonal antibody is aseptically filtered and lyophilized from PBS with no carrier proteins or preservatives added.

Reconstitution

Aseptically reconstitute this antigen affinity purified polyclonal antibody using sterile deionized water to a concentration >0.2 mg/ml.

Storage and Stability

The lyophilized antibody should be stored desiccated at -20°C. The reconstituted antibody can be stored for at least six weeks at 4°C. For long-term storage of the reconstituted antibody, aseptically aliquot into working volumes and store at -20°C in a manual defrost freezer. Avoid Repeated Freeze Thaw Cycles.

Reported Applications

ELISA Cap, WB

ELISA Sandwich: This antibody is useful as the capture antibody in a sandwich ELISA. The suggested coating concentration is 0.5-2.0 µg/ml. A suitable detection antibody is Anti-Human gAcrp30/Adipolean-Biotin (Antigen Aff. Pur.) (Leinco Prod. No.: A224) at a concentration of approximately 0.25-1.0 µg/ml. When used in conjunction with an optimal second step reagent such as UltraAvidin™- Horseradish Peroxidase (HRPO) (Leinco Prod. No.: A106) and a chromogenic substrate such as TMB HRPO Microwell Substrate Standard Kinetic One Component "Ready Use" (Leinco Prod. No.: T118), the sensitivity of this sandwich ELISA is 0.2-04 ng/well. A suggested standard for this assay is Recombinant Human gAcrp30 (Leinco Prod. No.: A222).

Western Blotting: To detect Human gAcrp30/Adipolean this polyclonal antibody can be used at a concentration of 0.1-0.2 µg/ml. This polyclonal antibody should be used in conjunction with compatible second-step reagents such as Donkey Anti-Goat IgG (H&L)-Horseradish Peroxidase (Leinco Prod. No.: G505) and a chromogenic substrate such as TMB HRPO Membrane/IHC Substrate UltraSensitive™ One Component "Ready Use" (Leinco Prod. No.: T343). The detection limit for Human gAcrp30/Adipolean is 1.5-3.0 ng/lane under either reducing or non-reducing conditions. The sensitivity of detection may increase up to 50 fold when a chemiluminescent substrate is used.

Each investigator should determine their own optimal working dilution for specific applications.

Country of Origin

USA