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AIF (Intermediate Domain) Blocking Peptide
Blocking Peptide
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Size


Prod. No.:A171
Conc.:0.2 mg/ml
Pkg. Size:50 µg
Storage:-20°C Detailed storage instructions below.
Description
Background:
AIF peptide is applicable as a control peptide for blocking antibody binding in Western blotting.

Amino Acid Location
This peptide sequence is located at amino acids 517 to 531 of Human AIF protein.
Formulation
This peptide is formulated in PBS pH 7.2 (0.01 M Sodium Phosphate, 0.13 M NaCl) containing 0.1% bovine serum albumin and 0.02% sodium azide.
Storage and Stability
Store this peptide in working aliquots at -20°C in a manual defrost freezer. Avoid Repeated Freeze Thaw Cycles.
Reported Applications
IHC (NBF/Par.), N, WB
Neutralization: The peptide may be used to neutralize the binding activity of Anti-Human Apoptosis Inducing Factor (Intermediate Domain) (AIF) (Leinco Prod. No.: A152) by incubation of an equal volume of the peptide and antibody for 30 minutes at 37°C for Western blotting experiments.
Each investigator should determine their own optimal working dilution for specific applications.
Country of Origin
USA
References
1. Susin SA, Lorenzo HK, Zamzami N, et al. Molecular characterization of mitochondrial apoptosis - inducing factor. Nature 1999;397:441-6
Related Products
Anti-Human Apoptosis Inducing Factor (Intermediate Domain) (AIF) (Leinco Prod. No.: A152)
AIF (NT) Blocking Peptide (Leinco Prod. No.: A172)
AIF (CT) Blocking Peptide (Leinco Prod. No.: A170)
Products are for research use only. Not for use in diagnostic or therapeutic procedures.
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