Amino-Terminal Enhancer of Split (NT) Blocking Peptide [A256] : Leinco Technologies, excellence in early discovery research

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Amino-Terminal Enhancer of Split (NT) Blocking Peptide

(AES, GRG, ESP1, TLE5 )

Blocking Peptide

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Size

50 µg ( $115.00 )

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Prod. No.:	A256
Conc.:	0.2 mg/ml
Pkg. Size:	50 µg
Storage:	-20°C Detailed storage instructions below.

Description

Background:

Amino-Terminal Enhancer of Split peptide is applicable as a control peptide for blocking antibody binding in Western blotting.

Amino Acid Location

16 amino acids near the amino terminus of human AES.

Formulation

This peptide is formulated in PBS pH 7.2 (0.01 M Sodium Phosphate, 0.13 M NaCl) containing 0.1% bovine serum albumin and 0.02% sodium azide.

Storage and Stability

Store this peptide in working aliquots at -20°C in a manual defrost freezer. Avoid Repeated Freeze Thaw Cycles.

Reported Applications

Indirect ELISA, N, WB

Neutralization: The peptide may be used to neutralize the binding activity of Anti-Human Amino-terminal Enhancer of Split (NT) (AES) (Leinco Prod. No.: A244) by incubation of an equal volume of the peptide and antibody for 30 minutes at 37°C for Western blotting experiments.

Each investigator should determine their own optimal working dilution for specific applications.

Country of Origin

USA

References

1. Fantuzzi G. Adipose tissue, adipokines, and inflammation. J. Allergy Clin. Immunol. 2005; 115:911-9.

2. Tsao T-S, Lodish HF, and Fruebis J. ACRP30, a new hormone controlling fat and glucose metabolism. Euro. J. Pharmacol. 2002; 440:213-21.

3. Wong GW, Wang J, Hug C, et al. A family of Acrp30/ adiponectin structural and functional paralogs. Proc. Natl. Acad. Sci. USA 2004; 101:10302-7.

4. Shapiro L and Scherer PE. The crystal structure of a complement-1q family protein suggests an evolutionary link to tumor necrosis factor. Curr. Biol. 1998; 8:335-8.