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Bcl-2 (Intermediate Domain) Blocking Peptide
Blocking Peptide
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Size


Prod. No.:B471
Conc.:0.2 mg/ml
Pkg. Size:50 µg
Storage:-20°C Detailed storage instructions below.
Description
Background:
Bcl-2 peptide is applicable as a control peptide for blocking antibody binding in Western blotting.

Amino Acid Location
14 amino acids near the center of human Bcl-2.
Formulation
This peptide is formulated in PBS pH 7.2 (0.01 M Sodium Phosphate, 0.13 M NaCl) containing 0.1% bovine serum albumin and 0.02% sodium azide.
Storage and Stability
Store this peptide in working aliquots at -20°C in a manual defrost freezer. Avoid Repeated Freeze Thaw Cycles.
Reported Applications
IHC (NBF/Par.), N, WB
Neutralization: The peptide may be used to neutralize the binding activity of Anti-Human Bcl-2 (Intermediate Domain) (Leinco Prod. No.: B445) by incubation of an equal volume of the peptide and antibody for 30 minutes at 37°C for Western blotting experiments.
Each investigator should determine their own optimal working dilution for specific applications.
Country of Origin
USA
References
1. Jarmuz A, Chester A, Bayliss J, et al. An anthropoid-specific locus of Orphan C to U RNA-editing enzymes on chromosome 22. Genomics 2002; 79:285-96.

2. Stremlau M, Owens CM, Perron MJ, et al. The cytoplasmic body component TRIM5α restricts HIV-1 infection in Old World monkeys. Nature 2004; 427:848-53.

3. Bieniasz PD. Intrinsic immunity: a front-line defense against viral attack. Nat Immunol. 2004; 5:1109-15.

4. Sheehy AM, Gaddis NC, Choi JD, et al. Isolation of a human gene that inhibits HIV-1 infection and is suppressed by the viral Vif protein. Nature 2002; 418:646-50.
Related Products
Anti-Human Bcl-2 (Intermediate Domain) (Leinco Prod. No.: B445)
Bcl-2 (NT) Blocking Peptide (Leinco Prod. No.: B472)
Anti-Human Bcl-2 (NT) (Leinco Prod. No.: B446)
Anti-Human Bcl-2 (Leinco Prod. No.: B589)
Anti-Human Bcl-2 (Leinco Prod. No.: B590)
Recombinant Human Bcl-2 (minus c-terminal) (Leinco Prod. No.: B548)
Anti-Human/Mouse Bcl-2 ( Antigen Aff. Pur. ) (Leinco Prod. No.: B642)
Products are for research use only. Not for use in diagnostic or therapeutic procedures.
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