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Recombinant Human Neuroserpin
Purified No Carrier Protein
(Serpin I1, Protease Inhibitor 12 (PI-12))
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Prod. No.:N175
SourceE. coli
Pkg. Size:25 µg, 1 mg
Storage:-20°C Detailed storage instructions below.
Description
Background:
Neuroserpin is an axonally secreted serine proteinase inhibitor that is expressed in neurons during embryogenesis and in the adult nervous system1 and is implicated in the regulation of proteases involved in processes such as synaptic plasticity, neuronal migration and axogenesis.2 Neuroserpin plays a neuroprotective role, by blocking the deleterious effects of proteolytic enzymes such as tissue plasminogen activator (tPA). A familial form of a neurodegenerative disease, termed familial encephalopathy with neuroserpin inclusion bodies, is caused by the intracellular polymerization and accumulation of mutated neuroserpin, leading to neuronal death and dementia.3

Source
E. coli
Molecular Weight
The molecular weight of Recombinant Human Neuroserpin is Mr 44.6 kDa.
State of Matter
Lyophilized
Purity
>96% by HPLC analysis
Storage and Stability
The lyophilized protein should be stored desiccated at -20°C. The reconstituted protein can be stored for at least one week at 4°C. For long-term storage of the reconstituted protein, aliquot into working volumes and store at -20°C in a manual defrost freezer. Avoid Repeated Freeze Thaw Cycles.
Endotoxin
<1.0 EU/µg
Biological Activity
The biological activity of Human Neuroserpin was determined by its ability to stimulate the proliferation of Rat C6 cells in the range of 0.5 - 0.6 ug/ml.
Each investigator should determine their own optimal working dilution for specific applications.
Amino Acid Sequence
MTGATFPEEA IADLSVNMYN RLRATGEDEN ILFSPLSIAL AMGMMELGAQ GSTQKEIRHS MGYDSLKNGE EFSFLKEFSN MVTAKESQYV MKIANSLFVQ NGFHVNEEFL QMMKKYFNAA VNHVDFSQNV AVANYINKWV ENNTNNLVKD LVSPRDFDAA TYLALINAVY FKGNWKSQFR PENTRTFSFT KDDESEVQIP MMYQQGEFYY GEFSDGSNEA GGIYQVLEIP YEGDEISMML VLSRQEVPLA TLEPLVKAQL VEEWANSVKK QKVEVYLPRF TVEQEIDLKD VLKALGITEI FIKDANLTGL SDNKEIFLSK AIHKSFLEVN EEGSEAAAVS GMIAISRMAV LYPQVIVDHP FFFLIRNRRT GTILFMGRVM HPETMNTSGH DFEEL


Country of Origin
USA
References
1. Peter Sonderegger et al. (1998) Ame Society of Biochem. Molec. Biol. 273: 2312
2. Birch NP. et al. (2002) J Neurochem. 80: 1406
3. Sonderegger P. et al. (2006) Front Bioscie. 11: 33
Products are for research use only. Not for use in diagnostic or therapeutic procedures.
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