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Recombinant Human 4-1BB Ligand (4-1BBL)
Purified No Carrier Protein
(Tumor Necrosis Factor (Ligand) Superfamily Member 9 (TNFSF9), CD137L)
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Prod. No.:F131
SourceE. coli
Pkg. Size:20 µg, 1 mg
Storage:-20°C to -70°C Detailed storage instructions below.
Description
Background:
4-1BB ligand (4-1BBL), also known as TNFSF9 and CD137L, is a type 2 transmembrane glycoprotein belonging to the TNF superfamily. It is a ligand for TNFRSF9, which is a co-stimulatory molecule in T lymphocytes. 4-1BBL and its receptor are involved in the antigen presentation process, generation of cytotoxic T cells and are required for the optimal CD8 responses in CD8 T cells (1). It is expressed on activated T and B cells, macrophages, dendritic cells, neurons and astrocytes (2-3). 4-1BBL also plays an important role in co-stimulation of IL-2 production (3). In addition, 4-1BBL is expressed in various carcinoma cell lines, and is thought to be involved in T cell-tumor cell interaction (4). One study reports the long-term remission of liver metastases in a mouse model involving the gene transfer of IL-12 and 4-1BBL (5).

Source
E. coli
Molecular Weight
The predicted molecular weight of Recombinant Human 4-1BBL is Mr 20.4 kDa.
State of Matter
Lyophilized
Formulation
This recombinant protein was 0.2 µm filtered and lyophilized from modified Dulbecco’s phosphate buffered saline (1X PBS) and NaCl; pH 7.2 – 7.3 with no calcium, magnesium, or preservatives.
Purity
>97% by SDS-PAGE and analyzed by silver stain.
Storage and Stability
This lyophilized protein is stable for six to twelve months when stored desiccated at -20°C to -70°C. After aseptic reconstitution and addition of a carrier protein (such as 0.1% BSA or HSA) this protein may be stored at -20°C to -70°C for up to 3 months in a manual defrost freezer. Avoid Repeated Freeze Thaw Cycles. See Product Insert for exact lot specific storage instructions.
Endotoxin
<1.0 EU/µg as determined by the LAL method
Biological Activity
The biological activity of Human 4-1BBL was determined by the dose-dependent induction of IL-8 by human PBMCs. The expected ED50 for this effect is 10 ng/ml.
Each investigator should determine their own optimal working dilution for specific applications.
Amino Acid Sequence
m regpelspdd paglldlrqg mfaqlvaqnv llidgplswy sdpglagvsl tgglsykedt kelvvakagv yyvffqlelr rvvagegsgs vslalhlqpl rsaagaaala ltvdlppass earnsafgfq grllhlsagq rlgvhlhtea rarhawqltq gatvlglfrv tpeipaglps prsehhhhhh



Country of Origin
USA
References
1. Watts, T. H. et al. (2002) J. Immunol. 168:4897
2. Watts, T. H. et al. (1995) J. Exp. Med. 181:985
3. Lotze, M. et al. (2001) Dendritic Cells: Biology and Clinical Applications (Academic
Press)
4. Tan, J. T. et al. (1999) J. Immunol. 163:4859
5. Martinet, O. et al. (2000) J. Natl. Cancer Inst. 92:931

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