NSO Cells

The predicted molecular weight of the reduced Human 4-1BBR monomer is Mr 44.8 kDa (monomer). However, the actual molecular weight, due to glycosylation, as observed by migration on SDS Page is Mr 50-65 kDa.

Lyophilized

This recombinant protein was 0.2 µm filtered and lyophilized from modified Dulbecco’s phosphate buffered saline (1X PBS) pH 7.2 – 7.3 with no calcium, magnesium, or preservatives.

>95% by SDS-PAGE and analyzed by silver stain.

The lyophilized protein should be stored desiccated at -20°C. The reconstituted protein can be stored for at least one week at 4°C. For long-term storage of the reconstituted protein, aliquot into working volumes and store at -20°C in a manual defrost freezer. Avoid Repeated Freeze Thaw Cycles.

<1.0 EU/µg as determined by the LAL method

The biological activity of Human 4-1BB Receptor is determined by its inhibitory effect of the 4-1BBL mediated stimulation of IL-8 production by human PBMC. About 90% of inhibition was seen using a concentration of 1 µg for both 4-1BBL and 4-1BBR.

The activity of the receptor was also measured by its ability to bind Recombinant Human 4-1BB Ligand in a functional ELISA. A coating concentration of 1 µg/ml was used for this assay.

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USA

Anti-Human 4-1BB Receptor-Biotin (Antigen Aff. Pur.) (Leinco Prod. No.: B392)
Anti-Human 4-1BB Receptor (Antigen Aff. Pur.) (Leinco Prod. No.: B391)