Recombinant Human CD137R (4-1BBR)

4-1BB Receptor, a member of the TNF superfamily of receptors, is mainly expressed on the surface of a variety of T cells, but also found in B cells, monocytes, and various transformed cell lines. 4-1BB Receptor binds to 4-1BBL to provide a co-stimulatory signal for T lymphocytes. Signaling by 4-1BB Receptor has been implicated in the antigen-presentation process and generation of cytotoxic T cells.

The biological activity of Human 4-1BB Receptor is determined by its inhibitory effect of the 4-1BBL mediated stimulation of IL-8 production by human PBMC. About 90% of inhibition was seen using a concentration of 1 µg for both 4-1BBL and 4-1BBR.</BR></BR>The activity of the receptor was also measured by its ability to bind Recombinant Human 4-1BB Ligand in a functional ELISA. A coating concentration of 1 µg/ml was used for this assay.

Fc Fusion Protein

lqdpcsn cpagtfcdnn rnqicspcpp nsfssaggqr tcdicrqckg vfrtrkecss tsnaecdctp gfhclgagcs mceqdckqgq eltkkgckdc cfgtfndqkr gicrpwtncs ldgksvlvng tkerdvvcgp spadlspgas svtppapare pghspqdieg rmdpkscdkt htcppcpape llggpsvflf ppkpkdtlmi srtpevtcvv vdvshedpev kfnwyvdgve vhnaktkpre eqynstyrvv svltvlhqdw lngkeykckv snkalpapie ktiskakgqp repqvytlpp srdeltknqv sltclvkgfy psdiavewes ngqpennykt tppvldsdgs fflyskltvd ksrwqqgnvf scsvmhealh nhytqkslsl spgkhhhhhh

Leu24

Lyophilized

The predicted molecular weight of the reduced Human 4-1BBR monomer is Mr 44.8 kDa. However, the actual molecular weight, due to glycosylation, as observed by migration on SDS-PAGE is Mr 50-65 kDa.

44.8

This recombinant protein was 0.2 µm filtered and lyophilized from modified Dulbecco’s phosphate buffered saline (1X PBS) pH 7.2 – 7.3 with no calcium, magnesium, or preservatives.

The lyophilized protein should be stored desiccated at -20°C. The reconstituted protein can be stored for at least one week at 4°C. For long-term storage of the reconstituted protein, aliquot into working volumes and store at -20°C in a manual defrost freezer. Avoid Repeated Freeze Thaw Cycles.

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