Recombinant Human Adiponectin (ACRP30)
Purified No Carrier Protein
(ADIPOQ, ACDC, ACRP30, APM-1, APM1, GBP28)
| Prod. No.: | A321 |
| Source | E. coli |
| Pkg. Size: | 1 mg, 25 µg, 100 µg |
| Storage: | -20°C Detailed storage instructions below. |
Description
Background:
Adiponectin, also known as Acrp30, is an adipocyte-derived protein with wide ranging paracrine and endocrine effects on metabolism and inflammation. It is induced during adipocyte differentiation, and its secretion is stimulated by insulin. It promotes adipocyte differentiation, fatty acid catabolism, and insulin sensitivity and is negatively correlated with obesity, type 2 diabetes, and atherogenesis. In this context, Adiponectin is an anti-inflammatory agent, but it exerts pro-inflammatory effects in nonmetabolic disorders such as rheumatoid arthritis and inflammatory bowel disease. Adiponectin interacts with the receptors AdipoR1 and AdipoR2, calreticulin, and Cadherin-13/T-Cadherin, as well as with several growth factors. Mature human Adiponectin shares 83% and 85% amino acid (aa) sequence identity with mouse and rat Adiponectin, respectively. The glycosylation of four hydroxylated lysine residues in the collagenous domain is required for the intracellular formation of HMW complexes. The various multimeric forms of Adiponectin exhibit distinct tissue specific and gender specific profiles and activities.
Source
E. coli
Molecular Weight
The predicted molecular weight of Recombinant Human ACRP30 is Mr 16.7 kDa.
State of Matter
Lyophilized
Formulation
Lyophilized from 10 mM Tris, pH 8.0 + 0.75 mM DTT at a concentration of 1 mg/ml.
Purity
>90% by SDS-PAGE and HPLC
Storage and Stability
The lyophilized protein should be stored desiccated at -20°C. The reconstituted protein can be stored for at least one week at 4°C. For long-term storage of the reconstituted protein, aliquot into working volumes and store at -20°C in a manual defrost freezer. Avoid Repeated Freeze Thaw Cycles.
Endotoxin
<0.125 EU/µg as determined by the LAL method
Biological Activity
ACRP-30 is fully biologically active when compared to standard. Activity is determined by the ability to inhibit the proliferation of mouse M1 cells. The expected ED50 for this effect is 1.0 – 2.5 μg/ml.
Each investigator should determine their own optimal working dilution for specific applications.
Amino Acid Sequence
MKGEPGEGAY VYRSAFSVGL ETYVTIPNMP IRFTKIFYNQ QNHYDGSTGK FHCNIPGLYY FAYHITVYMK DVKVSLFKKD KAMLFTYDQY QENNVDQASG SVLLHLEVGD QVWLQVYGEG ERNGLYADND NDSTFTGFLL YHDTN
Country of Origin
USA
References
1. Xu, A. et al. (2003) J. Clin. Invest. 112:91
2. Wang, Y. et al. (2002) J. Biol. Chem. 277:19521
3. Yamauchi, T. et al. (2002) Nat. Med. 8:1288
4. Berg, A. H. et al. (2001) Nat. Med. 7:947
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Products are for research use only. Not for use in diagnostic or therapeutic procedures.
