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Recombinant Human Activated Leukocyte Cell Adhesion Molecule (ALCAM)
Purified No Carrier Protein
(CD166, MEMD, SC-1/DM-GRASP/BEN, KG-CAM)
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Prod. No.:A347
SourceNSO Cells
Pkg. Size:100 µg, 1 mg
Storage:-20°C to -70°C Detailed storage instructions below.
Description
Background:
This protein is a member of the immunoglobulin gene superfamily expressed by leukocytes after cell activation and thymic epithelial cells. In the nomenclature of CD antigens this protein has been given the designation CD166. ALCAM is a ligand for the lymphocyte antigen CD6 but also engages in homophilic interactions. Mouse ALCAM shows an overall identity of 93 % with the human protein. ALCAM has been identified independently as MEMD. mRNA transcripts encoding murine ALCAM are expressed in the brain, lung, liver, and the kidney, as well as by activated leukocytes and a number of cell lines. A rat monoclonal antibody specific for murine ALCAM binds to both activated CD4(+) and CD8(+) T-cells. The antigen is expressed on natural killer cells, thymocytes, platelets, B-cells and T-cells after cell activation, eosinophils, fibroblasts, endothelial cells, keratinocytes, monocytes after cell activation, neurons. The ligand for CD166 is CD6.

Source
NSO Cells
Molecular Weight
The predicted molecular weight of Recombinant Human ALCAM is Mr 83.5 kDa (monomer). However, the actual molecular weight as observed by migration on SDS Page is Mr 120 kDa.
State of Matter
Lyophilized
Formulation
This recombinant protein was 0.2 µm filtered and lyophilized from modified Dulbecco’s phosphate buffered saline (1X PBS) pH 7.2 – 7.3 with no calcium, magnesium, or preservatives.
Purity
>95% by SDS-PAGE and analyzed by silver stain.
Storage and Stability
This lyophilized protein is stable for six to twelve months when stored desiccated at -20°C to -70°C. After aseptic reconstitution, this protein may be stored at 2°C to 8°C for one month or at -20°C to -70°C in a manual defrost freezer. Avoid Repeated Freeze Thaw Cycles. See Product Insert for exact lot specific storage instructions.
Endotoxin
<1.0 EU/µg as determined by the LAL method
Biological Activity
The biological activity of Human ALCAM was determined by its ability to block adhesion of HUT-78, a human cutaneous T cell lymphoma, to immobilized recombinant human CD6/Fc protein. Recombinant human ALCAM/Fc at 5 μg/ml (50 μl/well) blocked 100% of HUT-78 cell adhesion (1 x 106 cells/ml, 50 μl/well) to 10 μg/ml (100 μl/well, immobilized) recombinant human CD6/Fc.
Each investigator should determine their own optimal working dilution for specific applications.
Amino Acid Sequence
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Country of Origin
USA
Related Products
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Anti-Human ALCAM (Antigen Aff. Pur.) (Leinco Prod. No.: A388)
Anti-Human ALCAM-Biotin (Antigen Aff. Pur.) (Leinco Prod. No.: A402)
Anti-Human ALCAM-R-phycoerythrin (R-PE) (Leinco Prod. No.: A407)
Products are for research use only. Not for use in diagnostic or therapeutic procedures.
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