Recombinant Human ALCAM

Recombinant Human ALCAM

Product No.: A347

[product_table name="All Top" skus="A347"]

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Alternate Names
CD166, MEMD, SC-1/DM-GRASP/BEN, KG-CAM
Product Type
Recombinant Protein
Expression Host
NS0 Cells
Species
Human
Applications
ELISA Cap

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Product Size
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Background

This protein is a member of the immunoglobulin gene superfamily expressed by leukocytes after cell activation and thymic epithelial cells. In the nomenclature of CD antigens this protein has been given the designation CD166. ALCAM is a ligand for the lymphocyte antigen CD6 but also engages in homophilic interactions. Mouse ALCAM shows an overall identity of 93 % with the human protein. ALCAM has been identified independently as MEMD. mRNA transcripts encoding murine ALCAM are expressed in the brain, lung, liver, and the kidney, as well as by activated leukocytes and a number of cell lines. A rat monoclonal antibody specific for murine ALCAM binds to both activated CD4(+) and CD8(+) T-cells. The antigen is expressed on natural killer cells, thymocytes, platelets, B-cells and T-cells after cell activation, eosinophils, fibroblasts, endothelial cells, keratinocytes, monocytes after cell activation, neurons. The ligand for CD166 is CD6.

Protein Details

Purity
>95% by SDS-PAGE and analyzed by silver stain.
Endotoxin Level
<0.1 EU/µg as determined by the LAL method
Biological Activity
The biological activity of Human ALCAM was determined by its ability to block adhesion of HUT-78, a human cutaneous T cell lymphoma, to immobilized recombinant human CD6/Fc protein. Recombinant human ALCAM/Fc at 5 μg/ml (50 μl/well) blocked 100% of HUT-78 cell adhesion (1 x 10<sup>6</sup> cells/ml, 50 μl/well) to 10 μg/ml (100 μl/well, immobilized) recombinant human CD6/Fc.
Fusion Protein Tag
Fc Fusion Protein
Protein Accession No.
Amino Acid Sequence
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N-terminal Sequence Analysis
Trp28
State of Matter
Lyophilized
Predicted Molecular Mass
The predicted molecular weight of Recombinant Human ALCAM is Mr 83.5 kDa. However, the actual molecular weight as observed by migration on SDS Page is Mr 120 kDa.
Predicted Molecular Mass
83.5
Formulation
This recombinant protein was 0.2 µm filtered and lyophilized from modified Dulbecco’s phosphate buffered saline (1X PBS) pH 7.2 – 7.3 with no calcium, magnesium, or preservatives.
Storage and Stability
This lyophilized protein is stable for six to twelve months when stored desiccated at -20°C to -70°C. After aseptic reconstitution, this protein may be stored at 2°C to 8°C for one month or at -20°C to -70°C in a manual defrost freezer. Avoid Repeated Freeze Thaw Cycles. See Product Insert for exact lot specific storage instructions.
Country of Origin
USA
Shipping
Next Day Ambient
NCBI Gene Bank
Elisa Sandwich Protocol
IMPORTANT Use lot specific datasheet for all technical information pertaining to this recombinant protein.
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Prod No.
Description
A347
Products are for research use only. Not for use in diagnostic or therapeutic procedures.