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Recombinant Human Activation-Induced TNFR Receptor Ligand (AITRL)
Purified No Carrier Protein
(TNFSF18, GITRL, TL6, Activation-Induced TNFR Member Ligand )
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Prod. No.:A214
SourceSf 21 Insect Cells
Pkg. Size:25 µg, 1 mg
Storage:-20°C to -70°C Detailed storage instructions below.
Description
Background:
Activation-induced TNFR member ligand (AITRL) is a member of the TNF superfamily. It is expressed in endothelial cells and along with its receptor, AITR, seems to regulate interactions between endothelial cells and activated T lymphocytes. AITRL is a type II transmembrane protein containing 177 amino acid residues.

Source
Sf 21 Insect Cells
Molecular Weight
The predicted molecular weight of Recombinant Human AITRL is Mr 15 kDa. However, the actual molecular weight as observed by migration on SDS Page is Mr 14-16 kDa.
State of Matter
Lyophilized
Formulation
This recombinant protein was 0.2 µm filtered and lyophilized from modified Dulbecco’s phosphate buffered saline (1X PBS) pH 7.2 – 7.3 with no calcium, magnesium, or preservatives.
Purity
>97% by SDS-PAGE and analyzed by silver stain.
Storage and Stability
The lyophilized protein should be stored desiccated at -20°C. The reconstituted protein can be stored for at least one week at 4°C. For long-term storage of the reconstituted protein, aliquot into working volumes and store at -20°C in a manual defrost freezer. Avoid Repeated Freeze Thaw Cycles.
Endotoxin
<1.0 EU/µg as determined by the LAL method
Biological Activity
The biological activity of Human AITRL is determined by its ability to stimulate IL-8 production by human PBMC using a concentration range of 1.0-10.0 ng/ml. Results may vary with PBMC donors.
Each investigator should determine their own optimal working dilution for specific applications.
Amino Acid Sequence
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Country of Origin
USA
Products are for research use only. Not for use in diagnostic or therapeutic procedures.
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