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Recombinant Human Amphiregulin (AR)
Purified No Carrier Protein
(AREGB, CRDGF, MGC13647, SDGF)
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Prod. No.:A338
SourceE. coli
Pkg. Size:100 µg, 1 mg
Storage:-20°C to -70°C Detailed storage instructions below.
Description
Background:
Amphiregulin (AR), also known as AREGB, is a glycosylated, heparin binding protein and member of the EGF family of cytokines (1). AR is an autocrine growth factor as well as a mitogen, for astrocytes, Schwann cells and fibroblasts. The soluble form of AR is released by proteolytic cleavage. AR binds to the same receptors as EGF and TGF-α, albeit with lower affinity, and can functionally replace EGF and TGF-α in murine keratinocytes (2). Its expression can be induced by phorbol ester, estrogen, androgen and other EGFR ligands (3). In vitro, AR inhibits the growth of various human carcinoma cell lines; contrastingly, the proliferation of some tumor cell, fibroblasts and normal cells is enhanced by AR (4-6). Overexpression of AR is reported in human cancers of breast, colon, stomach and pancreas, as well as in psoriatic epidermis.

Source
E. coli
Molecular Weight
The predicted molecular weight of Recombinant Human Amphiregulin is Mr 11 kDa.
State of Matter
Lyophilized
Formulation
This recombinant protein was 0.2 µm filtered and lyophilized from modified Dulbecco’s phosphate buffered saline (1X PBS) pH 7.2 – 7.3 with no calcium, magnesium, or preservatives.
Purity
>97% by SDS-PAGE and analyzed by silver stain.
Storage and Stability
This lyophilized protein is stable for six to twelve months when stored desiccated at -20°C to -70°C. After aseptic reconstitution, this protein may be stored at 2°C to 8°C for one month or at -20°C to -70°C in a manual defrost freezer. Avoid Repeated Freeze Thaw Cycles. See Product Insert for exact lot specific storage instructions.
Endotoxin
<1.0 EU/µg as determined by the LAL method
Biological Activity
The biological activity of Human Amphiregulin was determined by its ability to stimulate the proliferation of a mouse fibroblast cell line, Balb/3T3 (Marquardt, H. et al., 1984, Science 223:1079). The expected ED50 is typically 5 - 15 ng/ml.
Each investigator should determine their own optimal working dilution for specific applications.
Amino Acid Sequence
svrveqvvkp pqnktesent sdkpkrkkkg gkngknrrnr kkknpcnaef qnfcihgeck yiehleavtc kcqqeyfger cgeksmkths midsslsk



Country of Origin
USA
References
1. Shoyab, M. et al. (1989) Science 243:1074
2. Johnson, G. R. et al. (1993) J. Biol. Chem. 268:2924
3. Thompson, S. et al. (1996) J. Biol. Chem. 271:17927
4. Johnson, G. R. et al. (1992) J. Cell Biol. 118:741
5. Salomon, D. S. et al. (1995) Breast Cancer Res. Treat. 33:103
6. Cook, P. W. et al. (1992) Cancer Res. 52:3324
Related Products
Recombinant Mouse Amphiregulin (Leinco Prod. No.: A354)
Anti-Human Amphiregulin (Antigen Aff. Pur.) (Leinco Prod. No.: A362)
Anti-Human Amphiregulin (Leinco Prod. No.: A372)
Anti-Human Amphiregulin-Biotin (Antigen Aff. Pur.) (Leinco Prod. No.: A395)
Products are for research use only. Not for use in diagnostic or therapeutic procedures.
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