Recombinant Human Beta Nerve Growth Factor (β-NGF)
Purified No Carrier Protein
(Beta-NGF, HSAN5, MGC161426, MGC161428, NGFB)
| Prod. No.: | N116 |
| Source | NSO Cells |
| Pkg. Size: | 1 mg, 100 µg |
| Storage: | -20°C to -70°C Detailed storage instructions below. |
Description
Background:
Nerve growth factor beta (NGFβ/beta-NGF) is a secreted protein of the neurotropin family and is important for the differentiation and survival of neurons and dermal keratinocytes (1). NGFβ is found in the hypothalamus, pituitary, thyroid gland, testes, epididymis, vascular smooth muscle cells, fibroblasts, mast cells and eosinophils. Glutamate, vitamin D3, IL-6, FGF basic, astrocyte specific IL-1, TNF alpha, PDGF and TGF beta up-regulate while GABAergic neuronal activity, glucocorticoids and Schwann cell-specific TGF beta down-regulate NFGβ. Signaling by NFGβ occurs through two receptors: TrKs and NGF receptor. It enhances the differentiation and survival of B lymphocytes (2) and also has a possible role in allergy and tissue repair (3).
Source
NSO Cells
Molecular Weight
The predicted molecular weight of Recombinant Human β-NGF is Mr 13.2 kDa.
State of Matter
Lyophilized
Formulation
This recombinant protein was 0.2 µm filtered and lyophilized from a sterile solution of 0.1M acetic acid.
Purity
>97% by SDS-PAGE and analyzed by silver stain.
Storage and Stability
This lyophilized protein is stable for six to twelve months when stored desiccated at -20°C to -70°C. After aseptic reconstitution, this protein may be stored at 2°C to 8°C for one month or at -20°C to -70°C in a manual defrost freezer. Avoid Repeated Freeze Thaw Cycles. See Product Insert for exact lot specific storage instructions.
Endotoxin
<1.0 EU/µg as determined by the LAL method
Biological Activity
The biological activity of Human β-NGF was determined by in a cell proliferation assay using a factor-dependent human erythroleukemic cell line, TF-1 (Kitamura, T. et al., 1989, J. Cell Physiol. 140:323-334). The ED50 for this effect is 0.8 - 1.5 ng/ml. β-NGF was also measured by its ability to support the survival and neurite outgrowth of cultured embryonic chick dorsal root ganglia (DRG). The expected ED50 0.5 - 1.0 ng/ml (Davies, A.M., 1989, “Neurotrophic Factor Bioassay Using Dissociated Neurons” in Nerve Growth Factors, R.A. Rush Ed., John Wiley and Sons, Ltd. pp. 95-109).
Each investigator should determine their own optimal working dilution for specific applications.
Amino Acid Sequence
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Country of Origin
USA
References
1. Schenck, K. et al. (2007) Eur. J. Oral Sci. 115:344
2. Otten, U. et al. (1989) Proc. Natl. Acad. Sci. (USA) 76:10059
3. Epplen, J. T. et al. (2008) BMC Med. Genet. 9:107
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Products are for research use only. Not for use in diagnostic or therapeutic procedures.
