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Recombinant Human CD23 (CD23)
Purified No Carrier Protein
(CD23 Antigen, Fc-epsilon-RII, Lymphocyte IgE receptor, BLAST-2)
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Prod. No.:C1591
SourceNSO Cells
Pkg. Size:50 µg, 1 mg
Storage:-20°C to -70°C Detailed storage instructions below.
Description
Background:
CD23, also known as Fc-ε-RII/BLAST-2, is a low affinity IgE Fc receptor which belongs to the C-type lectin structural family and plays a role in the regulation of IgE synthesis and IgE mediated activities (1). It is distinct from the high affinity IgE receptors found on basophils and mast cells, which mediate allergic reactions. CD23 is found both as a transmembrane receptor protein and in a soluble form, which is generated by proteolytic cleavage of membrane bound CD23 (2). It is expressed on most mature, conventional B cells (but not on peritoneal CD5+ B cells), and can also be found on the surface of T cells, macrophages, platelets, eosinophils and EBV transformed B lymphoblasts (3). Expression of CD23 has also been detected in neoplastic cells from cases of B cell chronic Lymphocytic leukemia (4). CD23 is expressed by B cells in the follicular mantle but not by proliferating germinal centre cells.

Source
NSO Cells
Molecular Weight
The predicted molecular weight of Recombinant Human CD23 is Mr 32 kDa. However, the actual molecular weight as observed by migration on SDS Page is Mr 41 kDa.
State of Matter
Lyophilized
Formulation
This recombinant protein was 0.2 µm filtered and lyophilized from modified Dulbecco’s phosphate buffered saline (1X PBS) pH 7.2 – 7.3 with no calcium, magnesium, or preservatives.
Purity
>90% by SDS-PAGE and analyzed by silver stain.
Storage and Stability
This lyophilized protein is stable for six to twelve months when stored desiccated at -20°C to -70°C. After aseptic reconstitution, this protein may be stored at 2°C to 8°C for one month or at -20°C to -70°C in a manual defrost freezer. Avoid Repeated Freeze Thaw Cycles. See Product Insert for exact lot specific storage instructions.
Endotoxin
<1.0 EU/µg as determined by the LAL method
Amino Acid Sequence
dtt qslkqleera arnvsqvskn leshhgdqma qksqstqisq eleelraeqq rlksqdlels wnlnglqadl ssfksqelne rneasdller lreevtklrm elqvssgfvc ntcpekwinf qrkcyyfgkg tkqwvharya cddmegqlvs ihspeeqdfl tkhashtgsw iglrnldlkg efiwvdgshv dysnwapgep tsrsqgedcv mmrgsgrwnd afcdrklgaw vcdrlatctp pasegsaesm gpdsrpdpdg rlptpsaplh shhhhhhhhh



Country of Origin
USA
References
1. Kijimoto-Ochiai, S. et al. (2002) Cell. Mol. Life Sci. 59:648
2. Visan, I. et al. (2003) Euro. J. Immunol. 33:1163
3. Abbas, A. K. et al. (2003) Cellular and Molecular Immunology, Saunders
4. Mossalayi, M. D et al. (1991) Annls. New York Acad. Sci. 628:362
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Anti-Human CD23-R-phycoerythrin (R-PE) (Leinco Prod. No.: C1533)
Recombinant Human CD23 (Soluble) (Leinco Prod. No.: C1318)
Anti-Human CD23 (Antigen Aff. Pur.) (Leinco Prod. No.: C1598)
Anti-Human CD23-Biotin (Antigen Aff. Pur.) (Leinco Prod. No.: C1600)
Anti-Human CD23 (Leinco Prod. No.: C1601)
Products are for research use only. Not for use in diagnostic or therapeutic procedures.
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