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Recombinant Human Cardiotrophin-1 (CT-1)
Purified No Carrier Protein
(CTF1)
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Prod. No.:C330
SourceE. coli
Pkg. Size:10 µg, 50 µg, 1 mg
Storage:-20°C to -70°C Detailed storage instructions below.
Description
Background:
Human CT-1 is a member of the IL-6 family of cytokines which also includes LIF, CNTF, OSM (Oncostatin M), IL-11, IL-6 and possibly NT-1/ BSF-3. CT-1 is a pleiotropic cytokine which signals through the LIF receptor and the gp130 receptor subunit. CT-1 has the ability to induce cardiac myocyte hypertrophy, and enhances the survival of cardiomyocyte and different neuronal populations. Biologically active human CT-1 is synthesized as a 201 amino acid polypeptide lacking a hydrophobic N-terminal secretion signal sequence.

Human and mouse CT-1 share 80% aa sequence identity and exhibit cross-species activity.

Source
E. coli
Molecular Weight
The predicted molecular weight of Recombinant Human CT-1 is Mr 21 kDa.
State of Matter
Solution
Formulation
This recombinant protein was 0.2 µm filtered and is supplied in a solution of 35% acetonitrile (CH3CN) and 0.1% trifluoroacetic acid (TFA).
Purity
>95% by SDS-PAGE and analyzed by silver stain.
Storage and Stability
This protein is stable for twelve months when stored at -20°C to -70°C. After thawing and addition of a carrier protein, this protein may be stored at 2°C to 8°C for one month or for long term storage aliquot and freeze at -20°C to -70°C in a manual defrost freezer. Avoid Repeated Freeze Thaw Cycles. See Product Insert for exact lot specific storage instructions.
Endotoxin
<1.0 EU/µg as determined by the LAL method
Biological Activity
The biological activity of Human CT-1 was determined in a cell proliferation assay using a factor-dependent human erythroleukemic cell line, TF-1 (Kitamura, T. et al., 1989, J. Cell Physiol. 140:323). The expected ED50 is typically 1 - 4 ng/ml.
Each investigator should determine their own optimal working dilution for specific applications.
Amino Acid Sequence
srregsled pqtdssvsll phleakirqt hslahlltky aeqllqeyvq lqgdpfglps fspprlpvag lsapapshag lpvherlrld aaalaalppl ldavcrrqae lnpraprllr rledaarqar algaaveall aalgaanrgp raeppaatas aasatgvfpa kvlglrvcgl yrewlsrteg dlgqllpggs a



Country of Origin
USA
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Products are for research use only. Not for use in diagnostic or therapeutic procedures.
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