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Recombinant Human Chemokine (C-C Motif) Ligand 1 (CCL1)
Purified No Carrier Protein
(SCYA1, I-309, T Cell Activation Gene 3 (TCA3), P500, SISe)
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Prod. No.:I-224
SourceE. coli
Pkg. Size:10 µg, 50 µg, 1 mg
Storage:-20°C to -70°C Detailed storage instructions below.
Description
Background:
Chemokine (C-C motif) ligand 1 (CCL1) is a small glycoprotein secreted by activated T cells that belongs to a family inflammatory cytokines known as chemokines.1 CCL1 attracts monocytes, NK cells, and immature B cells and dendritic cells by interacting with a cell surface chemokine receptor called CCR8.2 CCL1 may play a role in lymphocyte recruitment in bronchial asthma.3

Source
E. coli
Molecular Weight
The predicted molecular weight of Recombinant Human CCL1 is Mr 8.5 kDa.
State of Matter
Lyophilized
Formulation
This recombinant protein was lyophilized from a 0.2 μm filtered solution in 35% acetonitrile (CH3CN) and 0.1% trifluoroacetic acid (TFA).
Purity
>97% by SDS-PAGE and analyzed by silver stain.
Storage and Stability
This lyophilized protein is stable for six to twelve months when stored desiccated at -20°C to -70°C. After aseptic reconstitution, this protein may be stored at 2°C to 8°C for one month or at -20°C to -70°C in a manual defrost freezer. Avoid Repeated Freeze Thaw Cycles. See Product Insert for exact lot specific storage instructions.
Endotoxin
<1.0 EU/µg as determined by the LAL method
Biological Activity
The biological activity of Human I-309 was determined by by its ability to chemoattract mouse BW5147 cells or hCCR8 transfected BaF/3 cells. The expected ED50 for these effects are typically 3 - 9 ng/ml and 1.5 - 7.5 ng/ml, respectively.
Each investigator should determine their own optimal working dilution for specific applications.


Country of Origin
USA
References
1. Krangel et al. (1992) Proceedings of the National Acad of Sci. 89: 2950
2. Roos et al. (1997) J Biol Chem. 272: 17251
3. Teran LM et al. (2006) Eur Respir J. 28: 59
Related Products
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Anti-Mouse CCL1 (Antigen Aff. Pur.) (Leinco Prod. No.: C1483)
Anti-Mouse CCL1-Biotin (Antigen Aff. Pur.) (Leinco Prod. No.: T504)
Anti-Human CCL1 (Leinco Prod. No.: I-643)
Anti-Human CCL1-Biotin (Antigen Aff. Pur.) (Leinco Prod. No.: I-177)
Recombinant Mouse CCL1 (Leinco Prod. No.: T410)
Products are for research use only. Not for use in diagnostic or therapeutic procedures.
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