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Recombinant Human Chemokine (C-X-C Motif) Ligand 10 (CXCL10)
Purified No Carrier Protein
(C7, IFI10, INP10, IP-10, SCYB10, Crg-2, GIP-10, Mob-1)
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Prod. No.:I-202
SourceE. coli
Pkg. Size:10 µg, 50 µg, 1 mg
Storage:-20°C to -70°C Detailed storage instructions below.
Description
Background:
Chemokine ligand 10 (CXCL10), also known as IP-10 and CRG-2, is a small cytokine belonging to the CXC chemokine family. Binding of this protein to the cell surface chemokine receptor CXCR3, results in pleiotropic effects, including stimulation of monocytes, natural killer and T cell migration and modulation of adhesion molecule expression. CXCL10 is secreted by several cell types in response to IFN-γ including monocytes, endothelial cells and fibroblasts (1). CXCL10 has been attributed to several roles, such as chemoattraction for monocytes/macrophages, T cells, NK cells, and dendritic cells, promotion of T cell adhesion to endothelial cells, antitumor activity and inhibition of bone marrow colony formation and angiogenesis (2-3). Additionally, it is angiostatic and mitogenic for vascular smooth muscle cells. CXCL10 plays a role in the regulation of tubular cell proliferation following renal ischemia-reperfusion injury (4). CXCL10 shows anti-microbial and anti-bacterial activity (5-6).

Source
E. coli
Molecular Weight
The predicted molecular weight of Recombinant Human CXCL10 is Mr 8.7 kDa.
State of Matter
Lyophilized
Formulation
This recombinant protein was 0.2 µm filtered and lyophilized from modified Dulbecco’s phosphate buffered saline (1X PBS) pH 7.2 – 7.3 with no calcium, magnesium, or preservatives.
Purity
>97% by SDS-PAGE and analyzed by silver stain.
Storage and Stability
This lyophilized protein is stable for six to twelve months when stored desiccated at -20°C to -70°C. After aseptic reconstitution, this protein may be stored at 2°C to 8°C for one month or at -20°C to -70°C in a manual defrost freezer. Avoid Repeated Freeze Thaw Cycles. See Product Insert for exact lot specific storage instructions.
Endotoxin
<1.0 EU/µg as determined by the LAL method
Biological Activity
The biological activity of Human IP-10 was determined by its ability to chemoattract T-lymphocytes cultured in the presence of IL-2 for 21 days or by mouse BaF/3 cells transfected with hCXCR3. The expected ED50 for these effects are typically 0.02 - 0.06 μg/ml and 0.015 - 0.075 μg/ml, respectively.
Each investigator should determine their own optimal working dilution for specific applications.
Amino Acid Sequence
mvplsrtvrc tcisisnqpv nprsleklei ipasqfcprv eiiatmkkkg ekrclnpesk aiknllkavs kerskrsp



Country of Origin
USA
References
1. Luster, A. D. et al. (1985) Nature 315:672
2. Dufour, J. H. et al. (2002) J. Immunol. 168
3. Angiolillo, A. L. et al. (1995) J. Exp. Med. 182:155
4. Kaneko, S. et al. (2008) Nephron Exp. Nephrol. 109:c29
5. Yang, D. et al. (2003) J. Leukoc. Biol. 74:448
6. Egesten, A. et al. (2007) J. Infect. Dis. 195:684

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Products are for research use only. Not for use in diagnostic or therapeutic procedures.
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