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Recombinant Human Chemokine (C-X-C motif) Ligand 1 (CXCL1)
Purified No Carrier Protein
(GRO1, GROa, MGSA, MGSA Alpha, NAP-3, SCYB1, FSP)
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Prod. No.:G125
SourceE. coli
Pkg. Size:10 µg, 50 µg, 1 mg
Storage:-20°C to -70°C Detailed storage instructions below.
Description
Background:
Chemokine (C-X-C motif) ligand 1 (CXCL1) is a small cytokine with mitogenic properties belonging to the CXC chemokine family that was previously called GRO1 oncogene, GROα, KC, Neutrophil-activating protein 3 (NAP-3) and melanoma growth stimulating activity, alpha (MSGA-α).1 CXCL1 is expressed by macrophages, neutrophils and epithelial cells.2 CXCL1 plays a role in spinal cord development by inhibiting the migration of oligodendrocyte precursors and is involved in the processes of angiogenesis, inflammation, wound healing.3,4

Source
E. coli
Molecular Weight
The predicted molecular weight of Recombinant Human CXCL1 is Mr 7.9 kDa.
State of Matter
Lyophilized
Formulation
This recombinant protein was lyophilized from a 0.2 μm filtered solution in 35% acetonitrile (CH3CN) and 0.1% trifluoroacetic acid (TFA).
Purity
>97% by SDS-PAGE and analyzed by silver stain.
Storage and Stability
This lyophilized protein is stable for six to twelve months when stored desiccated at -20°C to -70°C. After aseptic reconstitution, this protein may be stored at 2°C to 8°C for one month or at -20°C to -70°C in a manual defrost freezer. Avoid Repeated Freeze Thaw Cycles. See Product Insert for exact lot specific storage instructions.
Endotoxin
<1.0 EU/µg as determined by the LAL method
Biological Activity
The biological activity of Human GRO/MGSA was determined by its ability to induce myeloperoxidase release from cytochalasin treated neutrophils (Schröder, J. et al., 1987, J. Immunol. 139:3474), or chemotaxis of mouse BaF/3 cells transfected with hCXCR2. The expected ED50 for these effects are typically 0.15 - 0.3 μg/ml or 1 - 4 ng/ml, respectively.
Each investigator should determine their own optimal working dilution for specific applications.
Amino Acid Sequence
asvate lrcqclqtlq gihpkniqsv nvkspgphca qteviatlkn grkaclnpas pivkkiiekm lnsdksn



Country of Origin
USA
References
1. Sager R et al. (1990) Proc. Natl. Acad. Sci. U.S.A. 87: 7732
2. Grotendorst GR et al. (1990) Mol. Cell. Biol. 10: 5596
3. Miller RH et al. (2002) Cell 110: 373
4. Richmond A et al. (2000) J. Invest. Dermatol. 115: 234
Related Products
Recombinant Mouse KC (Leinco Prod. No.: K114)
Recombinant Rat GROα/KC (Leinco Prod. No.: G126)
Rat CINC-1/CXCL1 ELISA Development Kit (Leinco Prod. No.: C1608)
Human GRO alpha/CXCL1 ELISA Development Kit (Leinco Prod. No.: G718)
Products are for research use only. Not for use in diagnostic or therapeutic procedures.
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