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Recombinant Human Erythropoietin Receptor (Epo R)
Purified No Carrier Protein
(EP, MGC138142)
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Prod. No.:E225
SourceNSO Cells
Pkg. Size:50 µg, 1 mg
Storage:-20°C to -70°C Detailed storage instructions below.
Description
Background:
The erythropoietin receptor is a transmembrane protein belonging to the member of the cytokine receptor family. The receptor is tyrosine phosphorylated upon binding by erythropoietin and associates with and activates the tyrosine kinase, JAK2, which activates different intracellular pathways including: Ras/MAP kinase, phosphatidylinositol 3-kinase and STAT transcription factors. EPO-R transmits signals important for both proliferation and differentiation along the erythroid lineage.1 Defects in the erythropoietin receptor may produce erythroleukemia and familial erythrocytosis.

Source
NSO Cells
Molecular Weight
The predicted molecular weight of Recombinant Human Epo R is Mr 51.4 kDa. However, the actual molecular weight as observed by migration on SDS Page is Mr 55-60 kDa.
State of Matter
Lyophilized
Formulation
This recombinant protein was 0.2 µm filtered and lyophilized from modified Dulbecco’s phosphate buffered saline (1X PBS) pH 7.2 – 7.3 with no calcium, magnesium, or preservatives.
Purity
>90% by SDS-PAGE and analyzed by silver stain.
Storage and Stability
This lyophilized protein is stable for six to twelve months when stored desiccated at -20°C to -70°C. After aseptic reconstitution, this protein may be stored at 2°C to 8°C for one month or at -20°C to -70°C in a manual defrost freezer. Avoid Repeated Freeze Thaw Cycles. See Product Insert for exact lot specific storage instructions.
Endotoxin
<0.1 ng per 1 µg as determined by the LAL method
Biological Activity
The biological activity of Human Epo R was determined by its ability to inhibit Epodependent proliferation of TF1 human erythroleukemic cells. Kitamura, T. et al. (1989) J. Cell Physiol. 140:323. The expected ED50 is 1 - 4 ng/ml in the presence of 0.2 U/ml of rhEpo.
Each investigator should determine their own optimal working dilution for specific applications.
Amino Acid Sequence
apppnlpdpk feskaallaa rgpeellcft erledlvcfw eeaasagvgp gnysfsyqle depwklcrlh qaptargavr fwcslptadt ssfvplelrv taasgapryh rvihinevvl ldapvglvar ladesghvvl rwlpppetpm tshiryevdv sagngagsvq rveilegrte cvlsnlrgrt rytfavrarm aepsfggfws awsepvsllt psdldpkscd kthtcppcpa pellggpsvf lfppkpkdtl misrtpevtc vvvdvshedp evkfnwyvdg vevhnaktkp reeqynstyr vvsvltvlhq dwlngkeykc kvsnkalpap iektiskakg qprepqvytl ppsrdeltkn qvsltclvkg fypsdiavew esngqpenny kttppvldsd gsfflysklt vdksrwqqgn vfscsvmhea lhnhytqksl slspgk



Country of Origin
USA
References
1. Siren AL et al. (2001) Proc Natl Acad Sci USA 98: 4044
2. Haroon ZA et al. (2003) Am J Pathol 163: 993
Related Products
Recombinant Human Erythropoietin Receptor (Leinco Prod. No.: E195)
Anti-Human Erythropoietin Receptor (Leinco Prod. No.: E245)
Anti-Human Erythropoietin Receptor-Biotin (Antigen Aff. Pur.) (Leinco Prod. No.: E277)
Anti-Human Epo R-R-phycoerythrin (R-PE) (Leinco Prod. No.: E303)
Anti-Human Epo R-Fluorescein (FITC) (Leinco Prod. No.: E302)
Products are for research use only. Not for use in diagnostic or therapeutic procedures.
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