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Recombinant Human Fas Ligand (FasL)
Purified No Carrier Protein
(TNFSF6, CD95L, Apo I Ligand, APTL, FASLG, APT1LG1, CD178)
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Prod. No.:F115
SourceCHO
Pkg. Size:10 µg, 1 mg
Storage:-20°C to -70°C Detailed storage instructions below.
Description
Background:
Fas ligand (FasL), also known as TNFSF6, is a type II transmembrane protein that belongs to the tumor necrosis factor (TNF) family and is involved in membrane mediated apoptosis. The specific receptor for FasL is Fas (CD95, Apo-1). FasL is primarily expressed on activated T cells and NK cells, while Fas is expressed on various types of cells. Ligation of Fas by FasL triggers activation of the caspase cascade. Functional impairment of the Fas/FasL system is associated with the development and progression of malignancies. Tumor cells frequently exhibit de novo expression of FasL, which plays a significant role in local tissue destruction, metastatic spread and immune escape of the tumor cells (1). The apoptosis of lymphocytes, which occurs in autoimmune diseases, is usually induced by the Fas/FasLsystem, therefore, FasL is regarded as a potential target for immunotherapy (2). One study has suggested a correlation between high levels of FasL expression in the cornea and successful corneal transplantation (3).

Source
CHO
Molecular Weight
The predicted molecular weight of Recombinant Human FasL is Mr 18 kDa. However, the actual molecular weight as observed on polyacrylimide gels is Mr 26 - 28 kDa.
State of Matter
Lyophilized
Formulation
This recombinant protein was 0.2 µm filtered and lyophilized from modified Dulbecco’s phosphate buffered saline (1X PBS) pH 7.2 – 7.3 with no calcium, magnesium, or preservatives.
Purity
>95% by SDS-PAGE and analyzed by silver stain.
Storage and Stability
This lyophilized protein is stable for six to twelve months when stored desiccated at -20°C to -70°C. After aseptic reconstitution, this protein may be stored at 2°C to 8°C for one month or at -20°C to -70°C in a manual defrost freezer. Avoid Repeated Freeze Thaw Cycles. See Product Insert for exact lot specific storage instructions.
Endotoxin
<1.0 EU/µg as determined by the LAL method
Biological Activity
The biological activity of Human Soluble Fas Ligand is determined by its ability to induce cytotoxicity in Jurkat cells. The expected ED50 for this effect is <1.0 - 3.0 ng/ml, in the presence of 10 µg/ml of a cross-linking antibody.
Each investigator should determine their own optimal working dilution for specific applications.
Amino Acid Sequence
mpspppek kelrkvahlt gksnsrsmpl ewedtygivl lsgvkykkgg lvinetglyf vyskvyfrgq scnnlplshk vymrnskypq dlvmmegkmm sycttgqmwa rssylgavfn ltsadhlyvn vselslvnfe esqtffglyk lhhhhhh



Country of Origin
USA
References
1. Igney, F. H. et al. (2005) Cancer Immunol. Immunother. 54:1127
2. Ottmar, J. et al. (2008) Cell Communication and Signaling 6:11
3. Stuart, P. et al. (1997) J. Clin. Investigation 99:396

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Products are for research use only. Not for use in diagnostic or therapeutic procedures.
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