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Recombinant Human Fibroblast Growth Factor Receptor 1 Alpha (FGF R1α (IIIb))
Purified No Carrier Protein
(CEK, FLG, OGD, FLT2, KAL2, BFGFR, CD331, FGFBR, HBGFR, N-SAM, FLJ99988, FGFR1)
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Prod. No.:F1041
SourceNSO Cells
Pkg. Size:50 µg, 1 mg
Storage:-20°C to -70°C Detailed storage instructions below.
Description
Background:
Fibroblast growth factor receptor-1 alpha (FGFR1-alpha) is an isoform of FGFR1 which is a receptor tyrosine kinase. FGFR1-alpha is expressed in prostate luminal epithelial cells.1 FGFR1alpha signals are posteriorizing factors that control node regression and posterior embryonic development.2

FGFR1-alpha is a splice variant of FGFR1 containing the first immunoglobulin-like domain, whereas FGFR1-beta lacks this domain.2 The presence or absence of this N-terminal domain influences the affinity of the receptor towards aFGF and heparin.3

FGFR1-alpha is expressed in prostate luminal epithelial cells, whereas FGFR1-beta is expressed in basal epithelial cells and smooth muscle cells.

Source
NSO Cells
Molecular Weight
The predicted molecular weight of Recombinant Human FGF R1α (IIIb) is Mr 66 kDa (monomer). However, the actual molecular weight as observed by migration on SDS Page is Mr 110-120 kDa.
State of Matter
Lyophilized
Formulation
This recombinant protein was 0.2 µm filtered and lyophilized from modified Dulbecco’s phosphate buffered saline (1X PBS) pH 7.2 – 7.3 with no calcium, magnesium, or preservatives.
Purity
>95% by SDS-PAGE and analyzed by silver stain.
Storage and Stability
This lyophilized protein is stable for six to twelve months when stored desiccated at -20°C to -70°C. After aseptic reconstitution, this protein may be stored at 2°C to 8°C for one month or at -20°C to -70°C in a manual defrost freezer. Avoid Repeated Freeze Thaw Cycles. See Product Insert for exact lot specific storage instructions.
Endotoxin
<1.0 EU/µg as determined by the LAL method
Amino Acid Sequence
rpsptlpeq aqpwgapvev esflvhpgdl lqlrcrlrdd vqsinwlrdg vqlaesnrtr itgeevevqd svpadsglya cvtsspsgsd ttyfsvnvsd alpssedddd dddssseeke tdntkpnpva pywtspekme kklhavpaak tvkfkcpssg tpnptlrwlk ngkefkpdhr iggykvryat wsiimdsvvp sdkgnytciv eneygsinht yqldvversp hrpilqaglp anktvalgsn vefmckvysd pqphiqwlkh ievngskigp dnlpyvqilk hsginssdae vltlfnvtea qsgeyvckvs nyigeanqsa wltvtrpale erpavmtspl yleiegrdmd pkscdkthtc ppcpapellg gpsvflfppk pkdtlmisrt pevtcvvvdv shedpevkfn wyvdgvevhn aktkpreeqy nstyrvvsvl tvlhqdwlng keykckvsnk alpapiekti skakgqprep qvytlppsrd eltknqvslt clvkgfypsd iavewesngq pennykttpp vldsdgsffl yskltvdksr wqqgnvfscs vmhealhnhy tqkslslspg k



Country of Origin
USA
References
1. Moscatelli D et al. (2007) Prostate 67: 115
2. Deng CX et al. (1999) Dev Biol. 208: 293
Related Products
Recombinant Human FGF R1α (IIIc) (Leinco Prod. No.: F1042)
Products are for research use only. Not for use in diagnostic or therapeutic procedures.
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