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Recombinant Human Glial Cell Line-Derived Neurotropic Factor Receptor Alpha 1 (GFRα-1)
Purified No Carrier Protein
(GFRA1, GDNFR, GDNFRA, GFR-ALPHA-1, MGC23045, RET1L, RETL1, TRNR1)
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Prod. No.:G653
SourceNSO Cells
Pkg. Size:100 µg, 1 mg
Storage:-20°C to -70°C Detailed storage instructions below.
Description
Background:
GDNF family receptor alpha 1, also known as GFRA1 is a glycosylphosphatidylinositol(GPI)-linked cell surface receptor for both GDNF and NTN, and mediates activation of the RET tyrosine kinase receptor. GFRalpha1 functions as a ligand-induced cell adhesion molecule (LICAM) to establish precise synaptic contacts and induce presynaptic differentiation.1

Source
NSO Cells
Molecular Weight
The predicted molecular weight of Recombinant Human GFRα-1 is Mr 73.3 kDa (monomer). However, the actual molecular weight as observed by migration on SDS Page is Mr 88-95 kDa.
State of Matter
Lyophilized
Formulation
This recombinant protein was 0.2 µm filtered and lyophilized from modified Dulbecco’s phosphate buffered saline (1X PBS) pH 7.2 – 7.3 with no calcium, magnesium, or preservatives.
Purity
>90% by SDS-PAGE and analyzed by silver stain.
Storage and Stability
This lyophilized protein is stable for six to twelve months when stored desiccated at -20°C to -70°C. After aseptic reconstitution, this protein may be stored at 2°C to 8°C for one month or at -20°C to -70°C in a manual defrost freezer. Avoid Repeated Freeze Thaw Cycles. See Product Insert for exact lot specific storage instructions.
Endotoxin
<1.0 EU/µg as determined by the LAL method
Biological Activity
The biological activity of Human GFRα-1 was determined by its ability to bind recombinant human GDNF. Immobilized recombinant human GFRα-1/Fc at 2 μg/ml (100 μl/well) can bind recombinant human GDNF with a linear range at 0.78 - 5 ng/ml in a functional ELISA assay.
Each investigator should determine their own optimal working dilution for specific applications.
Amino Acid Sequence
ae vsggdrldcv kasdqclkeq scstkyrtlr qcvagketnf slasgleakd ecrsamealk qkslyncrck rgmkkekncl riywsmyqsl qgndlledsp yepvnsrlsd ifrvvpfisv ehipkgnncl daakacnldd ickkyrsayi tpcttsvsnd vcnrrkchka lrqffdkvpa khsygmlfcs crdiacterr rqtivpvcsy eerekpncln lqdscktnyi crsrladfft ncqpesrsvs sclkenyadc llaysgligt vmtpnyidss slsvapwcdc snsgndleec lkflnffkdn tclknaiqaf gngsdvtvwq pafpvqttta ttttalrvkn kplgpagsen eipthvlppc anlqaqklks nvsgnthlci sngnyekegl gasshittki egrmdpkscd kthtcppcpa pellggpsvf lfppkpkdtl misrtpevtc vvvdvshedp evkfnwyvdg vevhnaktkp reeqynstyr vvsvltvlhq dwlngkeykc kvsnkalpap iektiskakg qprepqvytl ppsrdeltkn qvsltclvkg fypsdiavew esngqpenny kttppvldsd gsfflysklt vdksrwqqgn vfscsvmhea lhnhytqksl slspgkhhhh hh



Country of Origin
USA
References
1. Ibáñez CF. et al. (2007)Nat Neurosci. 10 : 293
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Products are for research use only. Not for use in diagnostic or therapeutic procedures.
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