Product Datasheet
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Recombinant Human Heregulin 1-β1 (HRG1-β1)
Purified No Carrier Protein
(Neuregulin1-β1 [NRG-β1], HRG, Breast Cancer Cell Differentiation Factor p45, Acetylcholine Receptor Inducing Activity [ARIA], Glial Factor, Neu Differentiation Factor)
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Prod. No.:H1420
SourceE. coli
Pkg. Size:50 µg, 1 mg
Storage:-20°C to -70°C Detailed storage instructions below.
Description
Background:
Neuregulin/Heregulin is a family of related growth factors that are differentially spliced variants of four genes: NRG1, NRG2, NRG3 and NRG4. The longest forms of NRG contains several different modular domains and tissue-specific splicing results in many NRG isoforms containing different sets of these domains. Alternative splicing produces two types of EGF domain, designated a and b. HRG-Beta1 contains an Ig domain and an EGF domain necessary for direct binding to receptor tyrosine kinases erbB-3 and erbB-4. HRG-Beta1 binding causes erbB-3 and erbB-4 to dimerize with erbB-2 (Her2/neu) and thereby regulates the phosphorylation of erbB-2 tyrosines. HRG-Beta1 stimulates proliferation and motility of breast cancer cells and plays a role in wound healing by stimulating epidermal migration and differentiation of epidermal cells and by stimulating the expression of integrins in the epidermis.

Source
E. coli
Molecular Weight
The predicted molecular weight of Recombinant Human HRG1-Beta1 is Mr 8 kDa.
State of Matter
Lyophilized
Formulation
This recombinant protein was lyophilized from a 0.2 μm filtered solution in 35% acetonitrile (CH3CN) and 0.1% trifluoroacetic acid (TFA).
Purity
>97% by SDS-PAGE and analyzed by silver stain.
Storage and Stability
This lyophilized protein is stable for six to twelve months when stored desiccated at -20°C to -70°C. After aseptic reconstitution, this protein may be stored at 2°C to 8°C for one month or at -20°C to -70°C in a manual defrost freezer. Avoid Repeated Freeze Thaw Cycles. See Product Insert for exact lot specific storage instructions.
Endotoxin
<1.0 EU/µg as determined by the LAL method
Biological Activity
The biological activity of Human HRG-Beta1 was determined by its ability to stimulate the proliferation of human MCF-7 cells grown under serum-free conditions (Karey, K. P. et. al., Cancer Research, 1988, 48:4083). The expected ED50 for this effect is typically 0.5 - 2 ng/ml.
Each investigator should determine their own optimal working dilution for specific applications.
Amino Acid Sequence
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Country of Origin
USA
Products are for research use only. Not for use in diagnostic or therapeutic procedures.
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