NSO Cells

The predicted molecular weight of Recombinant Human ICAM-1 is Mr 76 kDa (monomer). However, the actual molecular weight as observed by migration on SDS Page is Mr 110-125 kDa.

Lyophilized

This recombinant protein was 0.2 µm filtered and lyophilized from modified Dulbecco’s phosphate buffered saline (1X PBS) pH 7.2 – 7.3 with no calcium, magnesium, or preservatives.

>95% by SDS-PAGE and analyzed by silver stain.

This lyophilized protein is stable for six to twelve months when stored desiccated at -20°C to -70°C. After aseptic reconstitution, this protein may be stored at 2°C to 8°C for one month or at -20°C to -70°C in a manual defrost freezer. Avoid Repeated Freeze Thaw Cycles. See Product Insert for exact lot specific storage instructions.

<1.0 EU/µg as determined by the LAL method

The biological activity of Human ICAM-1 was determined by the ability of the immobilized protein to support the adhesion of HSB2 cells, a human peripheral blood acute lymphoblastic leukemia cell line. When 5 x 10⁴ cells/well are added to recombinant human ICAM/Fc coated plates (12.5 μg/ml with 100 μl/well), approximately 60 - 70% will adhere after PMA stimulation for 1 hour at RT.

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USA

Anti-Human ICAM-1 Purified (Leinco Prod. No.: C167)
Anti-Human ICAM-1-Fluorescein (FITC) (Leinco Prod. No.: C169)
Anti-Human ICAM-1-R-phycoerythrin/Cy5 (Leinco Prod. No.: C346)
Recombinant Rat ICAM-1 (Leinco Prod. No.: I-571)
Anti-Rat ICAM-1 (Leinco Prod. No.: I-682)
Anti-Human ICAM-1 (Leinco Prod. No.: I-698)
Anti-Rat ICAM-1 (Antigen Aff. Pur.) (Leinco Prod. No.: I-727)
Anti-Human ICAM-1 (Antigen Aff. Pur.) (Leinco Prod. No.: I-734)
Anti-Mouse ICAM-1 (Antigen Aff. Pur.) (Leinco Prod. No.: I-741)
Anti-Rat ICAM-1-Biotin (Antigen Aff. Pur.) (Leinco Prod. No.: I-786)
Anti-Human ICAM-1-Biotin (Antigen Aff. Pur.) (Leinco Prod. No.: I-794)
Recombinant Mouse ICAM-1 (Leinco Prod. No.: I-587)
Anti-Mouse ICAM-1-Allophycocyanin (Leinco Prod. No.: C354)
Anti-Mouse ICAM-1-Fluorescein (Leinco Prod. No.: C269)
Anti-Mouse ICAM-1-Biotin (Leinco Prod. No.: C268)
Anti-Mouse ICAM-1-R-phycoerythrin (Leinco Prod. No.: C270)
Anti-Human ICAM-1 Purified Low Endotoxin Functional Formulation (Leinco Prod. No.: C372)
Anti-Mouse ICAM-1 Low Endotoxin Functional Formulation (Leinco Prod. No.: C391)
Anti-Human ICAM-1-Allophycocyanin (APC) (Leinco Prod. No.: C323)
Anti-Human ICAM-1-Biotin (Leinco Prod. No.: C168)
PN:
Anti-Human ICAM-1-R-phycoerythrin (R-PE) (Leinco Prod. No.: C170)
Anti-Mouse ICAM-1 Purified (Leinco Prod. No.: C267)