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Recombinant Mouse Interferon Gamma (IFN-γ)
Purified No Carrier Protein
(Immune Interferon, Type II Interferon, T Cell Interferon, MAF, IFNG, IFG, IFI)
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Prod. No.:I-217
SourceE. coli
Pkg. Size:100 µg, 1 mg
Storage:-20°C to -70°C Detailed storage instructions below.
Description
Background:
Interferon-gamma (IFN-γ) or type II interferon is a dimerized soluble cytokine that is the only member of the type II class of interferons.1 It is a cytokine critical for innate and adaptive immunity against viral and intracellular bacterial infections and for tumor control. IFNG is produced predominantly by natural killer (NK) and natural killer T (NKT) cells as part of the innate immune response, and by CD4 and CD8 cytotoxic T lymphocyte (CTL) effector T cells once antigen-specific immunity develops.2 IFN-γ has antiviral, immunoregulatory, and anti-tumour properties.3

Source
E. coli
Molecular Weight
The predicted molecular weight of Recombinant Mouse IFN-γ is Mr 15.6 kDa. However, the actual molecular weight as observed by migration on SDS Page is Mr 11-12 kDa.
State of Matter
Lyophilized
Formulation
This recombinant protein was 0.2 µm filtered and lyophilized from modified Dulbecco’s phosphate buffered saline (1X PBS) with trehalose pH 7.2 – 7.3 with no calcium, magnesium, or preservatives.
Purity
>97% by SDS-PAGE and analyzed by silver stain.
Storage and Stability
This lyophilized protein is stable for six to twelve months when stored desiccated at -20°C to -70°C. After aseptic reconstitution, this protein may be stored at 2°C to 8°C for one month or at -20°C to -70°C in a manual defrost freezer. Avoid Repeated Freeze Thaw Cycles. See Product Insert for exact lot specific storage instructions.
Endotoxin
<1.0 EU/µg as determined by the LAL method
Biological Activity
The biological activity of Mouse Interferon-Gamma was measured in an anti-viral assay using L-929 cells infected with EMC virus.1 The expected ED50 for this effect is 0.3 - 0.9 ng/ml.
Each investigator should determine their own optimal working dilution for specific applications.
Amino Acid Sequence
mhgtviesl eslnnyfnss gidveekslf ldiwrnwqkd gdmkilqsqi isfylrlfev lkdnqaisnn isvieshlit tffsnskakk dafmsiakfe vnnpqvqrqa fnelirvvhq llpesslrkr krsrc



Country of Origin
USA
References
1. Goeddel DV et al. (1982) Nature 298: 859
2. Wilson CB et al. (2007) Adv. Immunol. 96: 41
3. Hume DA et al. (2004) J Leukoc Biol. 75: 163
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PN:
Products are for research use only. Not for use in diagnostic or therapeutic procedures.
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