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Recombinant Human Interleukin-1 Receptor Type I (IL-1 RI)
Purified No Carrier Protein
(IL1R1, CD121A, D2S1473, IL-1R-Alpha, IL1R, IL1RA, P80)
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Prod. No.:I-556
SourceNSO Cells
Pkg. Size:100 µg, 1 mg
Storage:-20°C to -70°C Detailed storage instructions below.
Description
Background:
Interleukin-1 receptor, type I (IL1R1), also known as CD121a, is a transmembrane glycoprotein and member of the IL-1 receptor family. As important mediators involved in many immune and inflammatory responses, the agonists IL-1α and IL-1β bind to IL1R1, leading to the signal transduction and activation of NF-κ B. IL1R1 also forms a ternary complex containing IL1RAP, TOLLIP, MYD88, and IRAK1 or IRAK2 which is indispensible for the signaling processes (1). IL1R1 has been shown to interact with PIK3R1, Myd88 and IL1RAP (2-4). It is expressed predominantly by T cells, fibroblasts and endothelial cells.

Source
NSO Cells
Molecular Weight
The predicted molecular weight of Recombinant Human IL-1 RI is Mr 36 kDa. However, the actual molecular weight as observed by migration on SDS Page is Mr 55 kDa.
State of Matter
Lyophilized
Formulation
This recombinant protein was 0.2 µm filtered and lyophilized from modified Dulbecco’s phosphate buffered saline (1X PBS) pH 7.2 – 7.3 with no calcium, magnesium, or preservatives.
Purity
>97% by SDS-PAGE and analyzed by silver stain.
Storage and Stability
This lyophilized protein is stable for six to twelve months when stored desiccated at -20°C to -70°C. After aseptic reconstitution, this protein may be stored at 2°C to 8°C for one month or at -20°C to -70°C in a manual defrost freezer. Avoid Repeated Freeze Thaw Cycles. See Product Insert for exact lot specific storage instructions.
Endotoxin
<1.0 EU/µg as determined by the LAL method
Amino Acid Sequence
dkckereeki ilvssaneid vrpcplnpne hkgtitwykd dsktpvsteq asrihqhkek lwfvpakved sghyycvvrn ssyclrikis akfvenepnl cynaqaifkq klpvagdggl vcpymeffkn ennelpklqw ykdckpllld nihfsgvkdr livmnvaekh rgnytchasy tylgkqypit rviefitlee nkptrpvivs panetmevdl gsqiqlicnv tgqlsdiayw kwngsvided dpvlgedyys venpankrrs tlitvlnise iesrfykhpf tcfaknthgi daayiqliyp vt



Country of Origin
USA
References
1. Burns, K. et al. (2006) Curr. Biol. 16:2265
2. Reddy, S. A. et al. (1995) J. Biol. Chem. 272:29167
3. Burns, K. et al. (2000) Nat. Cell Biol. 2:346
4. Huang, J. et al. (1997) Proc. Natl. Acad. Sci. (USA) 94:12829
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Anti-Mouse IL-1 RI (Antigen Aff. Pur.) (Leinco Prod. No.: I-736)
Anti-Human IL-1 RI-Biotin (Antigen Aff. Pur.) (Leinco Prod. No.: I-766)
Anti-Mouse IL-1 RI-Biotin (Antigen Aff. Pur.) (Leinco Prod. No.: I-796)
Products are for research use only. Not for use in diagnostic or therapeutic procedures.
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