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Product Datasheet

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Donkey Anti-Human IgG, Fc (Gamma) Fragment Specific

DyLight^® 488

Secondary Antibody

Adsorbed Against: Ms., Bv., Hs.

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Size

0.5 mg ( $219.00 )

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Prod. No.:	H1495
Conc.:	0.5 - 1.0 mg/ml
Pkg. Size:	0.5 mg
Storage:	2-8°C Detailed storage instructions below.

Description

Specificity:

Donkey Whole Molecule Anti-Human IgG, Fc (Gamma) Fragment Specific recognizes Human IgG, Fc (Gamma) Fragment Specific. This secondary antibody was purified using antigen affinity chromatography and has been adsorbed against serum proteins and immunoglobulins to minimize species cross-reactivity.

Reporter Molecule:

DyLight® 488 conjugated antibodies absorb light maximally around 493 nm (± 4 nm) and fluoresce with a peak around 518 nm. They are brighter than Cy2 and FITC conjugates and similar in brightness to Alexa Fluor 488 conjugates, but typically give less background. DyLight® 488 conjugated to any antibody is recommended to maximize sensitivity for all immunofluorescence procedures requiring a green-fluorescing dye. DyLight® is a trademark of Thermo Fisher Scientific Inc. and its subsidiaries.

Host Species

Donkey

Immunogen

Purified Human IgG, Fc (Gamma) Fragment Specific (>98%)

Formulation

This secondary antibody conjugate is formulated in 0.01 M phosphate buffered saline (PBS) pH 7.4, 150 mM NaCl, 1% BSA and 0.09% sodium azide as a preservative.

Expiration Date

One year from date of receipt

Storage and Stability

This secondary antibody conjugate should be stored desiccated at 2-8°C.

Applications and Suggested Working Dilutions

It is recommended that working dilutions be prepared fresh daily.

FC: 1:100 - 1:800
IF: 1:100 - 1:800

Secondary antibody concentrations reconstituted with glycerol will be diluted by 50%. Therefore, the working dilution may be one half of what is recommended.

Country of Origin

USA