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Product Datasheet

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Donkey Anti-Goat IgG (H&L)

DyLight^® 488

Secondary Antibody

Adsorbed Against: Ms., Hu., Rt., Rb., Hs., GP., CK., Sy. Hms.

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Size

0.5 mg ( $219.00 )

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Prod. No.:	G711
Conc.:	0.5 - 1.0 mg/ml
Pkg. Size:	0.5 mg
Storage:	2-8°C Detailed storage instructions below.

Description

Specificity:

Donkey Whole Molecule Anti-Goat IgG (H&L) recognizes Goat IgG (H&L). This secondary antibody was purified using antigen affinity chromatography and has been adsorbed against serum proteins and immunoglobulins to minimize species cross-reactivity.

Reporter Molecule:

DyLight® 488 conjugated antibodies absorb light maximally around 493 nm (± 4 nm) and fluoresce with a peak around 518 nm. They are brighter than Cy2 and FITC conjugates and similar in brightness to Alexa Fluor 488 conjugates, but typically give less background. DyLight® 488 conjugated to any antibody is recommended to maximize sensitivity for all immunofluorescence procedures requiring a green-fluorescing dye. DyLight® is a trademark of Thermo Fisher Scientific Inc. and its subsidiaries.

Host Species

Donkey

Immunogen

Purified Goat IgG (H&L) (>98%)

Formulation

This secondary antibody conjugate is formulated in 0.01 M phosphate buffered saline (PBS) pH 7.4, 150 mM NaCl, 1% BSA and 0.09% sodium azide as a preservative.

Expiration Date

One year from date of receipt

Storage and Stability

This secondary antibody conjugate should be stored desiccated at 2-8°C.

Applications and Suggested Working Dilutions

It is recommended that working dilutions be prepared fresh daily.

FC: 1:100 - 1:800
IF: 1:100 - 1:800

Secondary antibody concentrations reconstituted with glycerol will be diluted by 50%. Therefore, the working dilution may be one half of what is recommended.

Country of Origin

USA