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Product Datasheet

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Donkey F(ab')₂ Anti-Mouse IgG (H&L)

AMCA

Secondary Antibody

Adsorbed Against: Ms., Hu., Rt., Rb., Shp., Hs., GP., CK., Sy. Hms.

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Size

0.3 mg ( $145.00 )

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Prod. No.:	M1162
Conc.:	1.5 mg/ml
Pkg. Size:	0.3 mg
Storage:	2-8°C Detailed storage instructions below.

Description

Specificity:

Donkey F(ab')₂ Anti-Mouse IgG (H&L) recognizes Mouse IgG heavy and light chains. This secondary antibody was purified using antigen affinity chromatography and adsorbed against serum proteins and immunoglobulins to minimize species cross-reactivity.

Reporter Molecule:

AMCA is a small fluorescent organic molecule with an absorbance maximum of 350 nm and an emission maximum of 450 nm.

Preparation:

This F(ab')₂ secondary antibody was generated by optimal pepsin degestion of the antisera followed by antigen affinity chromatography to elimiate the presence of Fc fragment and whole molecules.

Host Species

Donkey

Immunogen

Purified Mouse IgG (H&L) (>98%)

Formulation

This secondary antibody is lyophilized from 0.01M phosphate buffered saline (PBS) pH 7.6 containing 0.25M NaCl, 15 mg/ml IgG free BSA and 0.05% sodium azide as a preservative.

Reconstitution

The lyophilized secondary antibody AMCA conjugate should be reconstituted in 0.25 ml of deionized water. Gently vortex until the solution is clear. Centrifuge the conjugate if not completely clear and free of particulates. After reconstitution, add an equal volume of ACS grade glycerol bringing the final concentration to 50% v/v.

Expiration Date

One year from date of reconstitution

Storage and Stability

This lyophilized secondary antibody conjugate should be stored desiccated at 2-8°C. The reconstituted AMCA conjugate containing glycerol should be stored at -20°C in a manual defrost freezer.

Applications and Suggested Working Dilutions

A working dilution of 1:50 - 1:200 is suitable for most applications.

Country of Origin

USA