Product Datasheet
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Donkey F(ab')2 Anti-Mouse IgG (H&L)
DyLight® 488
Secondary Antibody
Adsorbed Against: Hu., Rt., Rb., Bv., Shp., Hs., GP., CK., Sy. Hms.
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Size


Prod. No.:M1348
Conc.:0.5 - 1.0 mg/ml
Pkg. Size:0.5 mg
Storage:2-8°C Detailed storage instructions below.
Description
Specificity:
Donkey F(ab')2 Anti-Mouse IgG (H&L) recognizes Mouse IgG (H&L). This secondary antibody was purified using antigen affinity chromatography and has been adsorbed against serum proteins and immunoglobulins to minimize species cross-reactivity.

Reporter Molecule:
DyLight® 488 conjugated antibodies absorb light maximally around 493 nm (± 4 nm) and fluoresce with a peak around 518 nm. They are brighter than Cy2 and FITC conjugates and similar in brightness to Alexa Fluor 488 conjugates, but typically give less background. DyLight® 488 conjugated to any antibody is recommended to maximize sensitivity for all immunofluorescence procedures requiring a green-fluorescing dye. DyLight® is a trademark of Thermo Fisher Scientific Inc. and its subsidiaries.

Preparation:
This F(ab')2 secondary antibody was generated by optimal pepsin degestion of the antisera followed by antigen affinity chromatography to elimiate the presence of Fc fragment and whole molecules.
Host Species
Donkey
Immunogen
Purified Mouse IgG (H&L) (>98%)
Formulation
This secondary antibody conjugate is formulated in 0.01 M phosphate buffered saline (PBS) pH 7.4, 150 mM NaCl, 1% BSA and 0.09% sodium azide as a preservative.
Expiration Date
One year from date of receipt
Storage and Stability
This secondary antibody conjugate should be stored desiccated at 2-8°C.
Applications and Suggested Working Dilutions
It is recommended that working dilutions be prepared fresh daily.

FC: 1:100 - 1:800
IF: 1:100 - 1:800

Secondary antibody concentrations reconstituted with glycerol will be diluted by 50%. Therefore, the working dilution may be one half of what is recommended.
Country of Origin
USA
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Products are for research use only. Not for use in diagnostic or therapeutic procedures.
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