Product Datasheet
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Donkey F(ab')2 Anti-Mouse IgG (H&L)
DyLight® 550
Secondary Antibody
Adsorbed Against: Hu., Rt., Rb., Bv., Shp., Hs., GP., CK., Sy. Hms.
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Size


Prod. No.:M1349
Conc.:0.5 - 1.0 mg/ml
Pkg. Size:0.5 mg
Storage:2-8°C Detailed storage instructions below.
Description
Specificity:
Donkey F(ab')2 Anti-Mouse IgG (H&L) recognizes Mouse IgG (H&L). This secondary antibody was purified using antigen affinity chromatography and has been adsorbed against serum proteins and immunoglobulins to minimize species cross-reactivity.

Reporter Molecule:
DyLight® 550 conjugated antibodies absorb light maximally around 562 (± 4 nm) and fluoresce with a peak around 576 nm. They are brighter than TRITC conjugates, and they are equal in brightness to Cy3 and Alexa Fluor 555. All Dylight® 550 antibodies are recommended for maximum sensitivity in all immunofluorescence detection procedures within the orange-red portion of the visible spectrum. DyLight® is a trademark of Thermo Fisher Scientific Inc. and its subsidiaries.

Preparation:
This F(ab')2 secondary antibody was generated by optimal pepsin degestion of the antisera followed by antigen affinity chromatography to elimiate the presence of Fc fragment and whole molecules.
Host Species
Donkey
Immunogen
Purified Mouse IgG (H&L) (>98%)
Formulation
This secondary antibody conjugate is formulated in 0.01 M phosphate buffered saline (PBS) pH 7.4, 150 mM NaCl, 1% BSA and 0.09% sodium azide as a preservative.
Expiration Date
One year from date of receipt
Storage and Stability
This secondary antibody conjugate should be stored desiccated at 2-8°C.
Applications and Suggested Working Dilutions
It is recommended that working dilutions be prepared fresh daily.

FC: 1:100 - 1:800
IF: 1:100 - 1:800

Secondary antibody concentrations reconstituted with glycerol will be diluted by 50%. Therefore, the working dilution may be one half of what is recommended.
Country of Origin
USA
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Products are for research use only. Not for use in diagnostic or therapeutic procedures.
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