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Product Datasheet

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Goat F(ab')₂ Anti-Mouse IgG (H&L)

DyLight^® 680

Secondary Antibody

Adsorbed Against: Hu.

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Size

0.5 mg ( $110.00 )

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Prod. No.:	M1394
Conc.:	0.5 mg/ml
Pkg. Size:	0.5 mg
Storage:	2-8°C Detailed storage instructions below.

Description

Specificity:

Goat F(ab')₂ Anti-Mouse IgG (H&L) recognizes Mouse IgG (H&L). This secondary antibody was purified using antigen affinity chromatography and has been adsorbed against serum proteins and immunoglobulins to minimize species cross-reactivity.

Reporter Molecule:

DyLight® 680 conjugated antibodies absorb light maximally around 682 nm (± 4 nm) and fluoresce maximally around 715 nm. DyLight® 680 produces near-infrared (IR) fluorescence that replaces other near-IR dyes, including Cy5 and Alexa Fluor 680, and is ideal for multi-color applications including fluorescence microscopy, flow cytometry, ELISA. DyLight® is a trademark of Thermo Fisher Scientific Inc. and its subsidiaries.

Preparation:

This F(ab')₂ secondary antibody was generated by optimal pepsin degestion of the antisera followed by antigen affinity chromatography to elimiate the presence of Fc fragment and whole molecules.

Host Species

Goat

Immunogen

Purified Mouse IgG (H&L) (>98%)

Formulation

This DyLight^® 680 conjugate is formulated in 0.01 M phosphate buffered saline (PBS) pH 7.4, 150 mM NaCl, 1% BSA and 0.09% sodium azide as a preservative.

Expiration Date

One year from date of reconstitution

Storage and Stability

This DyLight^® 680 conjugate is stable when stored at 2-8°C. Do not freeze.

Applications and Suggested Working Dilutions

Secondary antibody concentrations reconstituted with glycerol will be diluted by 50%. Therefore, the working dilution may be one half of what is recommended.

Country of Origin

USA