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Product Datasheet

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Donkey F(ab')₂ Anti-Rabbit IgG (H&L)

Horseradish Peroxidase (HRPO)

Secondary Antibody

Adsorbed Against: Ms., Hu., Rt., Bv., Shp., Hs., GP., CK., Sy. Hms.

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Size

0.24 mg ( $150.00 )

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Prod. No.:	R1202
Conc.:	0.8 mg/ml
Pkg. Size:	0.24 mg

Description

Specificity:

Donkey F(ab')₂ Anti-Rabbit IgG (H&L) recognizes Rabbit IgG heavy and light chains. This secondary antibody was purified using antigen affinity chromatography and adsorbed against serum proteins and immunoglobulins to minimize species cross-reactivity.

Reporter Molecule:

Horseradish Peroxidase (HRPO) is an enzyme purified from horseradish root with a molecular weight of approximately 44 kDa.

Preparation:

This F(ab')₂ secondary antibody was generated by optimal pepsin degestion of the antisera followed by antigen affinity chromatography to elimiate the presence of Fc fragment and whole molecules.

Host Species

Donkey

Immunogen

Purified Rabbit IgG (H&L) (>98%)

Formulation

This secondary antibody is lyophilized from 0.01M phosphate buffered saline (PBS) pH 7.6 containing 0.25M NaCl, 15 mg/ml IgG free BSA. (Warning: Use of sodium azide as a preservative will inhibit the enzyme activity of horseradish peroxidase)

Expiration Date

One year from date of reconstitution

Storage and Stability

This lyophilized secondary antibody conjugate should be stored desiccated at 2-8°C. Caution: This horseradish peroxidase conjugate does not contain preservatives. To avoid microbial contamination, store the reconstituted conjugate containing glycerol at -20°C in a manual defrost freezer.

Country of Origin

USA