Donkey F(ab’)2 Anti-Rat IgG (H&L) (Adsorbed Against: Ms., Hu., Rb., Bv., Shp., Hs., GP., CK., Sy. Hms.) – Alk Phos

Donkey F(ab’)2 Anti-Rat IgG (H&L) (Adsorbed Against: Ms., Hu., Rb., Bv., Shp., Hs., GP., CK., Sy. Hms.) – Alk Phos

Product No.: R1229

[product_table name="All Top" skus="R1229"]

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Target
IgG (H&L)
Product Type
Secondary Antibody

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Product Size
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Antibody Details

Product Details

Reactive Species
Rat
Host Species
Donkey
Immunogen
Purified Rat IgG (H&L) (>98%)
Product Concentration
0.6 mg/ml
Formulation
This secondary antibody is lyophilized from 0.01M Tris buffered saline (TBS) pH 8.0 containing 0.25M NaCl, 15 mg/ml IgG free BSA and 0.05% sodium azide as a preservative.
Product Preparation
This F(ab')2 secondary antibody was generated by optimal pepsin degestion of the antisera followed by antigen affinity chromatography to elimiate the presence of Fc fragment and whole molecules.
Storage and Handling
This lyophilized secondary antibody conjugate should be stored desiccated at 2-8°C. The reconstituted Alkaline Phosphatase (Alk Phos) conjugate containing glycerol should be stored at -20°C in a manual defrost freezer.
Expiration Date
One year from date of reconstitution
Country of Origin
USA
Shipping
Next Day Ambient
Working Concentration
WB: 1:5,000; IHC 1:50,000
ELISA: 1:5,000; IHC 1:50,000
Each investigator should determine their own optimal working dilution for specific applications. See directions on lot specific datasheets, as information may periodically change.

Description

Specificity
Donkey F(ab')2 Anti-Rat IgG (H&L) recognizes Rat IgG heavy and light chains. This secondary antibody was purified using antigen affinity chromatography and adsorbed against serum proteins and immunoglobulins to minimize species cross-reactivity.

Antigen Details

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Products are for research use only. Not for use in diagnostic or therapeutic procedures.