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Product Datasheet

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Donkey F(ab')₂ Anti-Rat IgG (H&L)

DyLight^® 594

Secondary Antibody

Adsorbed Against: Ms., Hu., Rb., Bv., Shp., Hs., GP., CK., Sy. Hms.

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Size

0.5 mg ( $399.00 )

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Prod. No.:	R1305
Conc.:	0.5 - 1.0 mg/ml
Pkg. Size:	0.5 mg
Storage:	2-8°C Detailed storage instructions below.

Description

Specificity:

Donkey F(ab')₂ Anti-Rat IgG (H&L) recognizes Rat IgG (H&L). This secondary antibody was purified using antigen affinity chromatography and has been adsorbed against serum proteins and immunoglobulins to minimize species cross-reactivity.

Reporter Molecule:

DyLight® 594 antibody conjugates absorb light maximally around 593 nm (± 4 nm) and fluoresce with a peak around 618 nm. They are distinctly brighter than Alexa 594 conjugates, and much brighter and more water soluble than Texas Red conjugates. DyLight® is a trademark of Thermo Fisher Scientific Inc. and its subsidiaries.

Preparation:

This F(ab')₂ secondary antibody was generated by optimal pepsin degestion of the antisera followed by antigen affinity chromatography to elimiate the presence of Fc fragment and whole molecules.

Host Species

Donkey

Immunogen

Purified Rat IgG (H&L) (>98%)

Formulation

This secondary antibody conjugate is formulated in 0.01 M phosphate buffered saline (PBS) pH 7.4, 150 mM NaCl, 1% BSA and 0.09% sodium azide as a preservative.

Expiration Date

One year from date of receipt

Storage and Stability

This secondary antibody conjugate should be stored desiccated at 2-8°C.

Applications and Suggested Working Dilutions

It is recommended that working dilutions be prepared fresh daily.

FC: 1:100 - 1:800
IF: 1:100 - 1:800

Secondary antibody concentrations reconstituted with glycerol will be diluted by 50%. Therefore, the working dilution may be one half of what is recommended.

Country of Origin

USA