Product Datasheet
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Donkey F(ab')2 Anti-Rat IgG (H&L)
DyLight® 650
Secondary Antibody
Adsorbed Against: Hu., Rb., Bv., Shp., Hs., GP., CK., Sy. Hms.
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Size


Prod. No.:R1310
Conc.:0.5 - 1.0 mg/ml
Pkg. Size:0.5 mg
Storage:2-8°C Detailed storage instructions below.
Description
Specificity:
Donkey F(ab')2 Anti-Rat IgG (H&L) recognizes Rat IgG (H&L). This secondary antibody was purified using antigen affinity chromatography and has been adsorbed against serum proteins and immunoglobulins to minimize species cross-reactivity.

Reporter Molecule:
DyLight® 650 conjugated antibodies absorb light maximally around 652 nm (± 4 nm) and fluoresce maximally around 672 nm. They are brighter than Cy5 and Alexa 647 conjugates plus, they typically give less background than Alexa 647 conjugates. DyLight® 650 antibody conjugates are the best choice for far red-emitting conjugates and for multiple-labeling detection with a confocal microscope. DyLight® is a trademark of Thermo Fisher Scientific Inc. and its subsidiaries.

Preparation:
This F(ab')2 secondary antibody was generated by optimal pepsin degestion of the antisera followed by antigen affinity chromatography to elimiate the presence of Fc fragment and whole molecules.
Host Species
Donkey
Immunogen
Purified Rat IgG (H&L) (>98%)
Formulation
This secondary antibody conjugate is formulated in 0.01 M phosphate buffered saline (PBS) pH 7.4, 150 mM NaCl, 1% BSA and 0.09% sodium azide as a preservative.
Expiration Date
One year from date of receipt
Storage and Stability
This secondary antibody conjugate should be stored desiccated at 2-8°C.
Applications and Suggested Working Dilutions
It is recommended that working dilutions be prepared fresh daily.

FC: 1:100 - 1:800
IF: 1:100 - 1:800

Secondary antibody concentrations reconstituted with glycerol will be diluted by 50%. Therefore, the working dilution may be one half of what is recommended.
Country of Origin
USA
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Products are for research use only. Not for use in diagnostic or therapeutic procedures.
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