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Product Datasheet

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Goat F(ab')₂ Anti-Rabbit IgG (H&L)

DyLight^® 650

Secondary Antibody

Adsorbed Against: Hu.

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Size

0.5 mg ( $150.00 )

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Prod. No.:	R1336
Conc.:	1.0 mg/ml
Pkg. Size:	0.5 mg
Storage:	2-8°C Detailed storage instructions below.

Description

Specificity:

Goat F(ab')₂ Anti-Rabbit IgG (H&L) recognizes Rabbit IgG (H&L). This secondary antibody was purified using antigen affinity chromatography and has been adsorbed against serum proteins and immunoglobulins to minimize species cross-reactivity.

Reporter Molecule:

DyLight® 650 conjugated antibodies absorb light maximally around 652 nm (± 4 nm) and fluoresce maximally around 672 nm. They are brighter than Cy5 and Alexa 647 conjugates plus, they typically give less background than Alexa 647 conjugates. DyLight® 650 antibody conjugates are the best choice for far red-emitting conjugates and for multiple-labeling detection with a confocal microscope. DyLight® is a trademark of Thermo Fisher Scientific Inc. and its subsidiaries.

Preparation:

This F(ab')₂ secondary antibody was generated by optimal pepsin degestion of the antisera followed by antigen affinity chromatography to elimiate the presence of Fc fragment and whole molecules.

Host Species

Goat

Immunogen

Purified Rabbit IgG (H&L) (>98%)

Formulation

This DyLight^® 650 conjugate is formulated in 0.01 M phosphate buffered saline (PBS) pH 7.4, 150 mM NaCl, 1% BSA and 0.09% sodium azide as a preservative.

Expiration Date

One year from date of reconstitution

Storage and Stability

This DyLight^® 650 conjugate is stable when stored at 2-8°C. Do not freeze.

Applications and Suggested Working Dilutions

Secondary antibody concentrations reconstituted with glycerol will be diluted by 50%. Therefore, the working dilution may be one half of what is recommended.

Country of Origin

USA