Product Datasheet
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Goat F(ab')2 Anti-Rat IgG (H&L)
DyLight® 755
Secondary Antibody
Adsorbed Against: Ms., Hu.
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Size


Prod. No.:R1349
Pkg. Size:0.5 mg
Storage:2-8°C Detailed storage instructions below.
Description
Specificity:
Goat F(ab')2 Anti-Rat IgG (H&L) recognizes Rat IgG (H&L). This secondary antibody was purified using antigen affinity chromatography and has been adsorbed against serum proteins and immunoglobulins to minimize species cross-reactivity.

Reporter Molecule:
DyLight® 755 conjugated antibodies absorb light maximally around 754 nm (± 4 nm) and fluoresce maximally around 776 nm. DyLight® 750 has spectral properties that are very similar to other near-IR dyes, including Alexa Fluor 750, and has high solubility for maximal incorporation of the dye into the biomolecule of interest. DyLight® is a trademark of Thermo Fisher Scientific Inc. and its subsidiaries.

Preparation:
This F(ab')2 secondary antibody was generated by optimal pepsin degestion of the antisera followed by antigen affinity chromatography to elimiate the presence of Fc fragment and whole molecules.
Host Species
Goat
Immunogen
Purified Rat IgG (H&L) (>98%)
Formulation
This DyLight® 755 conjugate is formulated in 0.01 M phosphate buffered saline (PBS) pH 7.4, 150 mM NaCl, 1% BSA and 0.09% sodium azide as a preservative.
Expiration Date
One year from date of reconstitution
Storage and Stability
This DyLight® 755 conjugate is stable when stored at 2-8°C. Do not freeze.
Applications and Suggested Working Dilutions

Secondary antibody concentrations reconstituted with glycerol will be diluted by 50%. Therefore, the working dilution may be one half of what is recommended.
Country of Origin
USA
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Products are for research use only. Not for use in diagnostic or therapeutic procedures.
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