This F(ab')₂ secondary antibody was generated by optimal pepsin degestion of the antisera followed by antigen affinity chromatography to elimiate the presence of Fc fragment and whole molecules.

Donkey

Purified Chicken IgY/IgG (H&L) (>98%)

This secondary antibody is lyophilized from 0.01M phosphate buffered saline (PBS) pH 7.6 containing 0.25M NaCl, 15 mg/ml IgG free BSA. (Warning: Use of sodium azide as a preservative will inhibit the enzyme activity of horseradish peroxidase)

The lyophilized secondary antibody Horseradish Peroxidase (HRPO) conjugate should be reconstituted in 0.3 ml of deionized water. Gently vortex until the solution is clear. Centrifuge the conjugate if not completely clear and free of particulates. After reconstitution, add an equal volume of ACS grade glycerol bringing the final concentration to 50% v/v.

One year from date of reconstitution

This lyophilized secondary antibody conjugate should be stored desiccated at 2-8°C. Caution: This horseradish peroxidase conjugate does not contain preservatives. To avoid microbial contamination, store the reconstituted conjugate containing glycerol at -20°C in a manual defrost freezer.

It is recommended that working dilutions be prepared fresh daily.

WB: 1:5,000 - 1:100,000
WB Chemiluminescent: 1:10,000 - 1:200,000
ELISA: 1:5,000 - 1:100,000
IHC: 1:500 - 1:5,000

Secondary antibody concentrations reconstituted with glycerol will be diluted by 50%. Therefore, the working dilution may be one half of what is recommended.

USA