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Product Datasheet

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Donkey F(ab')₂ Anti-Chicken Igy/IgG (H&L)

Fluorescein (FITC)

Secondary Antibody

Adsorbed Against: Ms., Hu., Rt., Rb., Bv., Shp., Hs., GP., Sy. Hms.

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Size

0.3 mg ( $125.00 )

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Prod. No.:	C1205
Conc.:	1.5 mg/ml
Pkg. Size:	0.3 mg
Storage:	2-8°C Detailed storage instructions below.

Description

Specificity:

Donkey F(ab')₂ Anti-Chicken Igy/IgG (H&L) recognizes Chicken Igy/IgG heavy and light chains. This secondary antibody was purified using antigen affinity chromatography and adsorbed against serum proteins and immunoglobulins to minimize species cross-reactivity.

Reporter Molecule:

Fluorescein (FITC) is a small fluorescent organic molecule with a molecular weight of 473 daltons with an absorbance maximum of ~492 nm and an emission maximum of ~518 nm.

Preparation:

This F(ab')₂ secondary antibody was generated by optimal pepsin degestion of the antisera followed by antigen affinity chromatography to elimiate the presence of Fc fragment and whole molecules.

Host Species

Donkey

Immunogen

Purified Chicken Igy/IgG (H&L) (>98%)

Formulation

This secondary antibody is lyophilized from 0.01M phosphate buffered saline (PBS) pH 7.6 containing 0.25M NaCl, 15 mg/ml IgG free BSA and 0.05% sodium azide as a preservative.

Reconstitution

The lyophilized secondary antibody Fluorescein (FITC) conjugate should be reconstituted in 0.25 ml of deionized water. Gently vortex until the solution is clear. Centrifuge the conjugate if not completely clear and free of particulates. After reconstitution, add an equal volume of ACS grade glycerol bringing the final concentration to 50% v/v.

Expiration Date

One year from date of reconstitution

Storage and Stability

This lyophilized secondary antibody conjugate should be stored desiccated at 2-8°C. The reconstituted Fluorescein (FITC) conjugate containing glycerol should be stored at -20°C in a manual defrost freezer.

Applications and Suggested Working Dilutions

A working dilution of 1:50 - 1:200 is suitable for most applications.

Country of Origin

USA