General Western Blot Protocol
The Western blot is an analytical technique used to detect specific proteins in a sample. The technique uses gel electrophoresis to separate native or denatured proteins by the size of the polypeptide or by the 3-D structure of the protein. Leinco Technologies offers a wide range of both polyclonal and monoclonal antibodies against thousands of proteins which can be used for the western blot protocol.
This protocol provides an initial set of conditions; however, further optimization may be required on an individual basis.
Prepare Cell/Tissue Lysate(s)
Protein Transfer (using tank transfer method)