Indirect ELISA Protocol
Leinco Technologies validates many of their products using ELISA (Enzyme-linked immunosorbent assay) methods. An ELISA is used to detect the presence of an antibody or antigen in a sample. This tool is used heavily as a diagnostic tool in medicine but, is mainly used as a quality control test at Leinco Technologies.
This protocol provides an initial set of conditions; however, further optimization may be required on an individual basis. If there are any questions regarding the use of Leinco antibodies or proteins please call our technical support team at (800) 538-1145.
Coating the Plate with Antigen
Blocking the Plates
Incubate with Primary Antibody
Incubate with Secondary Antibody
Note: Do not use sodium azide in any buffers or solutions as sodium azide inactivates the horseradish-peroxidase enzyme. All solutions should be at ambient temperature prior to use.